A small-molecule targeting the microRNA binding domain of argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic leukemia cell line NB4

Silvia Masciarelli; Roberto Quaranta; Ilaria Iosue; Gianni Colotti; Fabrizio Padula; Greta Varchi; Francesco Fazi; Alberto Del Rio

doi:10.1021/cb500286b

A small-molecule targeting the microRNA binding domain of argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic leukemia cell line NB4

Silvia Masciarelli, Roberto Quaranta, Ilaria Iosue, Gianni Colotti, Fabrizio Padula, Greta Varchi, Francesco Fazi, Alberto Del Rio

Università Cattolica del Sacro Cuore

Risultato della ricerca: Contributo in rivista › Articolo in rivista

23 Citazioni (Scopus)

Abstract

Argonaute proteins are pivotal regulators of gene expression mediating miRNAs function. Modulating their activity would be extremely useful to elucidate the processes governing small-RNAs-guided gene silencing. We report the identification of a chemical compound able to compete with Argonaute 2 miRNAs binding, and we demonstrate that this functional inhibition determines effects similar to Argonaute 2 shRNA-mediated down-regulation, favoring granulocytic differentiation of the acute promyelocytic leukemia cell line NB4 in response to retinoic acid. © 2014 American Chemical Society.

Lingua originale	English
pagine (da-a)	1674-1679
Numero di pagine	6
Rivista	ACS Chemical Biology
Volume	9
DOI	https://doi.org/10.1021/cb500286b
Stato di pubblicazione	Pubblicato - 2014

Keywords

Argonaute Proteins
Cell Differentiation
Cell Line, Tumor
Humans
Leukemia, Promyelocytic, Acute
MicroRNAs
Small Molecule Libraries
Tretinoin

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10.1021/cb500286b

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title = "A small-molecule targeting the microRNA binding domain of argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic leukemia cell line NB4",

abstract = "Argonaute proteins are pivotal regulators of gene expression mediating miRNAs function. Modulating their activity would be extremely useful to elucidate the processes governing small-RNAs-guided gene silencing. We report the identification of a chemical compound able to compete with Argonaute 2 miRNAs binding, and we demonstrate that this functional inhibition determines effects similar to Argonaute 2 shRNA-mediated down-regulation, favoring granulocytic differentiation of the acute promyelocytic leukemia cell line NB4 in response to retinoic acid. {\textcopyright} 2014 American Chemical Society.",

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journal = "ACS Chemical Biology",

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T1 - A small-molecule targeting the microRNA binding domain of argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic leukemia cell line NB4

AU - Masciarelli, Silvia

AU - Quaranta, Roberto

AU - Iosue, Ilaria

AU - Colotti, Gianni

AU - Padula, Fabrizio

AU - Varchi, Greta

AU - Fazi, Francesco

AU - Del Rio, Alberto

PY - 2014

Y1 - 2014

N2 - Argonaute proteins are pivotal regulators of gene expression mediating miRNAs function. Modulating their activity would be extremely useful to elucidate the processes governing small-RNAs-guided gene silencing. We report the identification of a chemical compound able to compete with Argonaute 2 miRNAs binding, and we demonstrate that this functional inhibition determines effects similar to Argonaute 2 shRNA-mediated down-regulation, favoring granulocytic differentiation of the acute promyelocytic leukemia cell line NB4 in response to retinoic acid. © 2014 American Chemical Society.

AB - Argonaute proteins are pivotal regulators of gene expression mediating miRNAs function. Modulating their activity would be extremely useful to elucidate the processes governing small-RNAs-guided gene silencing. We report the identification of a chemical compound able to compete with Argonaute 2 miRNAs binding, and we demonstrate that this functional inhibition determines effects similar to Argonaute 2 shRNA-mediated down-regulation, favoring granulocytic differentiation of the acute promyelocytic leukemia cell line NB4 in response to retinoic acid. © 2014 American Chemical Society.

KW - Argonaute Proteins

KW - Cell Differentiation

KW - Cell Line, Tumor

KW - Humans

KW - Leukemia, Promyelocytic, Acute

KW - MicroRNAs

KW - Small Molecule Libraries

KW - Tretinoin

KW - Argonaute Proteins

KW - Cell Differentiation

KW - Cell Line, Tumor

KW - Humans

KW - Leukemia, Promyelocytic, Acute

KW - MicroRNAs

KW - Small Molecule Libraries

KW - Tretinoin

UR - http://hdl.handle.net/10807/147544

UR - http://pubs.acs.org/journal/acbcct

U2 - 10.1021/cb500286b

DO - 10.1021/cb500286b

M3 - Article

SN - 1554-8929

VL - 9

SP - 1674

EP - 1679

JO - ACS Chemical Biology

JF - ACS Chemical Biology

ER -

A small-molecule targeting the microRNA binding domain of argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic leukemia cell line NB4

Abstract

Keywords

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