TY - JOUR
T1 - A Reversed-Phase HPLC Fluorimetric Method for Simultaneous Determination of Homocysteine-Related Thiols in Different Body Fluids
AU - Persichilli, Silvia
AU - Gervasoni, Jacopo
AU - Castagnola, Massimo
AU - Zuppi, Cecilia
AU - Zappacosta, Bruno
PY - 2011
Y1 - 2011
N2 - Background: Low molecular weight thiols (cysteine, cysteinylglycine,
glutathione {[GSH] and homocysteine) are important intermediates in
different metabolic pathways. Glutathione has a relevant role as an
antioxidant in detoxification of toxic compounds and xenobiotics, and
homocysteine represents a risk factor for cardiovascular, neurological,
and congenital diseases. Homocysteine and GSH are metabolically related
in a pathway including cysteine and cysteinylglycine as intermediates.
For these reasons, determination of homocysteine and related thiols is
of great importance in the diagnosis of several diseases.
Methods: The measurement of these sulphur compounds can be performed by
using different methods, such as liquid or gas chromatography/mass
spectrometry, high-performance liquid chromatography (HPLC), or
capillary electrophoresis.
Results: This study describes an HPLC method coupled with fluorimetric
detection for the simultaneous determination of cysteine,
cysteinylglycine, GSH, and homocysteine in different biological fluids
(blood, saliva, urine, and cerebrospinal fluid [CSF]).
Conclusion: The comparison of the results obtained by other authors, as
well as the method validation and the analytical costs, indicate that
this HPLC method is particularly suitable for routine measurement of
thiols in different body fluids.}
AB - Background: Low molecular weight thiols (cysteine, cysteinylglycine,
glutathione {[GSH] and homocysteine) are important intermediates in
different metabolic pathways. Glutathione has a relevant role as an
antioxidant in detoxification of toxic compounds and xenobiotics, and
homocysteine represents a risk factor for cardiovascular, neurological,
and congenital diseases. Homocysteine and GSH are metabolically related
in a pathway including cysteine and cysteinylglycine as intermediates.
For these reasons, determination of homocysteine and related thiols is
of great importance in the diagnosis of several diseases.
Methods: The measurement of these sulphur compounds can be performed by
using different methods, such as liquid or gas chromatography/mass
spectrometry, high-performance liquid chromatography (HPLC), or
capillary electrophoresis.
Results: This study describes an HPLC method coupled with fluorimetric
detection for the simultaneous determination of cysteine,
cysteinylglycine, GSH, and homocysteine in different biological fluids
(blood, saliva, urine, and cerebrospinal fluid [CSF]).
Conclusion: The comparison of the results obtained by other authors, as
well as the method validation and the analytical costs, indicate that
this HPLC method is particularly suitable for routine measurement of
thiols in different body fluids.}
KW - HPLC
KW - HPLC
UR - http://hdl.handle.net/10807/5945
U2 - 10.1309/LM0IAH19RG58KBIQ
DO - 10.1309/LM0IAH19RG58KBIQ
M3 - Article
SN - 0007-5027
VL - 42
SP - 657
EP - 662
JO - Laboratory Medicine
JF - Laboratory Medicine
ER -