A rapid method for the extraction, enantiomeric separation and quantification of amphetamines in hair.

Sabina Strano Rossi, Francesco Botrè, Ana Bermejo, M. Jesus Tabernero

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

24 Citazioni (Scopus)

Abstract

This paper presents a rapid and sensitive method for the determination and chiral separation of amphetamines and related designer drugs in hair samples. The substances are extracted from hair matrix by a 30 min treatment with a saturated carbonate buffer at pH 10 under ultrasonication. A commercial chiral derivatizating agent, trifluoroacetyl-prolyl chloride, is then added to the solution that is directly extracted with hexane and subsequently analysed by GC/MS in SIM mode. R and S isomers of amphetamine, methamphetamine, MDA, MDMA and MDEA can be separated and detected with a limit of detection of 0.1 ng/mg for amphetamine, methamphetamine and MDA, and of 0.2 ng/mg for MDMA and MDEA. The method was then applied to twelve samples from suspected amphetamines abusers, showing the presence of both isomers of amphetamine and MDMA in one sample (27 and 1.5 ng/mg, respectively) and of MDMA in further eight samples, in concentrations ranging from traces to 2.7 ng/mg. No differences were observed in the disposition of different isomers in hair.
Lingua originaleEnglish
pagine (da-a)95-100
Numero di pagine6
RivistaForensic Science International
Volume2009
DOI
Stato di pubblicazionePubblicato - 2009

Keywords

  • Amphetamines, Designer drugs, Hair analysis, Chiral separation, Forensic Toxicology.

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