Abstract
There is a real medical need of new diagnostic tools for the early recognition of invasive Candida infections. We exploited a rather simple and rapid redox methodology to construct a bispecific monoclonal antibody (bsmAb) that combines a monoclonal antibody (mAb) directed against 1,3-β-D-glucan, a well-known, pan-fungal diagnostic biomarker, with a mAb recognizing MP65, a major immunogenic mannoprotein secreted by C.albicans and other Candida species. The bsmAb (MP65/bglu mAb) was successfully produced and purified at high yields and proved to bind and reveal simultaneously, with high sensitivity, the β- glucan and MP65 antigens in both purified and native forms. The MP65/bglu mAb is the first bispecific antibody generated against a fungal microorganism and may prove useful for the concurrent detection of different and clinically significant Candida biomarkers in patient sera.
Lingua originale | English |
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pagine (da-a) | e0148714-N/A |
Rivista | PLoS One |
Volume | 11 |
DOI | |
Stato di pubblicazione | Pubblicato - 2016 |
Keywords
- Agricultural and Biological Sciences (all)
- Animals
- Antibodies, Bispecific
- Antibodies, Fungal
- Antibodies, Monoclonal
- Antibody Specificity
- Antigens, Fungal
- Biochemistry, Genetics and Molecular Biology (all)
- Biomarkers
- Candida
- Candida albicans
- Candidiasis, Invasive
- Fungal Proteins
- Humans
- Immunodominant Epitopes
- Medicine (all)
- Membrane Glycoproteins
- Mice
- Serologic Tests
- beta-Glucans