A murine, bispecific monoclonal antibody simultaneously recognizing β-Glucan and MP65 determinants in Candida species

Roberto Cauda, Andrea Zito, Carla Bromuro, Giorgia Mandili, Paola Chiani, Alberto L. Horenstein, Fabio Malavasi, Antonio Cassone, Antonella Torosantucci

Risultato della ricerca: Contributo in rivistaArticolo in rivista

9 Citazioni (Scopus)

Abstract

There is a real medical need of new diagnostic tools for the early recognition of invasive Candida infections. We exploited a rather simple and rapid redox methodology to construct a bispecific monoclonal antibody (bsmAb) that combines a monoclonal antibody (mAb) directed against 1,3-β-D-glucan, a well-known, pan-fungal diagnostic biomarker, with a mAb recognizing MP65, a major immunogenic mannoprotein secreted by C.albicans and other Candida species. The bsmAb (MP65/bglu mAb) was successfully produced and purified at high yields and proved to bind and reveal simultaneously, with high sensitivity, the β- glucan and MP65 antigens in both purified and native forms. The MP65/bglu mAb is the first bispecific antibody generated against a fungal microorganism and may prove useful for the concurrent detection of different and clinically significant Candida biomarkers in patient sera.
Lingua originaleEnglish
pagine (da-a)e0148714-N/A
RivistaPLoS One
Volume11
DOI
Stato di pubblicazionePubblicato - 2016

Keywords

  • Agricultural and Biological Sciences (all)
  • Animals
  • Antibodies, Bispecific
  • Antibodies, Fungal
  • Antibodies, Monoclonal
  • Antibody Specificity
  • Antigens, Fungal
  • Biochemistry, Genetics and Molecular Biology (all)
  • Biomarkers
  • Candida
  • Candida albicans
  • Candidiasis, Invasive
  • Fungal Proteins
  • Humans
  • Immunodominant Epitopes
  • Medicine (all)
  • Membrane Glycoproteins
  • Mice
  • Serologic Tests
  • beta-Glucans

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