A fast chemiluminescent method for H2O2 measurement in exhaled breath condensate.

Bruno Zappacosta; Silvia Persichilli; Flaminio Mormile; Andrea Russo; Bruno Giardina; Pasquale De Sole

A fast chemiluminescent method for H2O2 measurement in exhaled breath condensate.

Bruno Zappacosta, Silvia Persichilli, Flaminio Mormile, Andrea Russo, Bruno Giardina, Pasquale De Sole

Risultato della ricerca: Contributo in rivista › Articolo in rivista

Abstract

Abstract BACKGROUND: Breath condensate can give useful information on volatile compounds produced at alveolar level. Actual concentration of H(2)O(2) in breath condensate is dependent on its production at alveolar level and on the efficacy of the detoxifying systems, catalase, glutathione peroxidase, etc. METHODS: In the present paper, a simple chemiluminescent method for the determination of the H(2)O(2) collected in exhaled breath is shown and data of both smokers and nonsmokers volunteers are presented. RESULTS: The chemiluminescent response is linear up to 100 micromol/l H(2)O(2). The analytical sensitivity is about 0.01 micromol/l. Most of the nonsmokers have a H(2)O(2) content lower than 0.05 micromol/l, while smokers have a content ranging from 0.1 to 0.6 micromol/l.

Lingua originale	English
pagine (da-a)	187-191
Numero di pagine	5
Rivista	Clinica Chimica Acta
Stato di pubblicazione	Pubblicato - 2001

Keywords

breath condensate
chemiluminescence
chemiluminescenza
cigarette smoking
condensato dell'espirato
fumo di sigaretta

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title = "A fast chemiluminescent method for H2O2 measurement in exhaled breath condensate.",

abstract = "Abstract BACKGROUND: Breath condensate can give useful information on volatile compounds produced at alveolar level. Actual concentration of H(2)O(2) in breath condensate is dependent on its production at alveolar level and on the efficacy of the detoxifying systems, catalase, glutathione peroxidase, etc. METHODS: In the present paper, a simple chemiluminescent method for the determination of the H(2)O(2) collected in exhaled breath is shown and data of both smokers and nonsmokers volunteers are presented. RESULTS: The chemiluminescent response is linear up to 100 micromol/l H(2)O(2). The analytical sensitivity is about 0.01 micromol/l. Most of the nonsmokers have a H(2)O(2) content lower than 0.05 micromol/l, while smokers have a content ranging from 0.1 to 0.6 micromol/l.",

keywords = "breath condensate, chemiluminescence, chemiluminescenza, cigarette smoking, condensato dell'espirato, fumo di sigaretta, breath condensate, chemiluminescence, chemiluminescenza, cigarette smoking, condensato dell'espirato, fumo di sigaretta",

author = "Bruno Zappacosta and Silvia Persichilli and Flaminio Mormile and Andrea Russo and Bruno Giardina and {De Sole}, Pasquale",

year = "2001",

language = "English",

pages = "187--191",

journal = "Clinica Chimica Acta",

issn = "0009-8981",

publisher = "Elsevier BV:PO Box 211, 1000 AE Amsterdam Netherlands:011 31 20 4853757, 011 31 20 4853642, 011 31 20 4853641, EMAIL: nlinfo-f@elsevier.nl, INTERNET: http://www.elsevier.nl, Fax: 011 31 20 4853598",

}

TY - JOUR

T1 - A fast chemiluminescent method for H2O2 measurement in exhaled breath condensate.

AU - Zappacosta, Bruno

AU - Persichilli, Silvia

AU - Mormile, Flaminio

AU - Russo, Andrea

AU - Giardina, Bruno

AU - De Sole, Pasquale

PY - 2001

Y1 - 2001

N2 - Abstract BACKGROUND: Breath condensate can give useful information on volatile compounds produced at alveolar level. Actual concentration of H(2)O(2) in breath condensate is dependent on its production at alveolar level and on the efficacy of the detoxifying systems, catalase, glutathione peroxidase, etc. METHODS: In the present paper, a simple chemiluminescent method for the determination of the H(2)O(2) collected in exhaled breath is shown and data of both smokers and nonsmokers volunteers are presented. RESULTS: The chemiluminescent response is linear up to 100 micromol/l H(2)O(2). The analytical sensitivity is about 0.01 micromol/l. Most of the nonsmokers have a H(2)O(2) content lower than 0.05 micromol/l, while smokers have a content ranging from 0.1 to 0.6 micromol/l.

AB - Abstract BACKGROUND: Breath condensate can give useful information on volatile compounds produced at alveolar level. Actual concentration of H(2)O(2) in breath condensate is dependent on its production at alveolar level and on the efficacy of the detoxifying systems, catalase, glutathione peroxidase, etc. METHODS: In the present paper, a simple chemiluminescent method for the determination of the H(2)O(2) collected in exhaled breath is shown and data of both smokers and nonsmokers volunteers are presented. RESULTS: The chemiluminescent response is linear up to 100 micromol/l H(2)O(2). The analytical sensitivity is about 0.01 micromol/l. Most of the nonsmokers have a H(2)O(2) content lower than 0.05 micromol/l, while smokers have a content ranging from 0.1 to 0.6 micromol/l.

KW - breath condensate

KW - chemiluminescence

KW - chemiluminescenza

KW - cigarette smoking

KW - condensato dell'espirato

KW - fumo di sigaretta

KW - breath condensate

KW - chemiluminescence

KW - chemiluminescenza

KW - cigarette smoking

KW - condensato dell'espirato

KW - fumo di sigaretta

UR - http://hdl.handle.net/10807/62968

M3 - Article

SN - 0009-8981

SP - 187

EP - 191

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

ER -

A fast chemiluminescent method for H2O2 measurement in exhaled breath condensate.

Abstract

Keywords

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