A circulating miRNA assay as a first-line test for prostate cancer screening

Evgeniya Sharova, Angela Grassi, Anna Marcer, Katia Ruggero, Francesco Pinto, Pierfrancesco Bassi, Paola Zanovello, Filiberto Zattoni, Donna M D'Agostino, Massimo Iafrate, Vincenzo Ciminale*

*Autore corrispondente per questo lavoro

Risultato della ricerca: Contributo in rivistaArticolo

37 Citazioni (Scopus)

Abstract

Prostate cancer (PCa) screening currently relies on prostate-specific antigen (PSA) testing and digital rectal examination. However, recent large-scale studies have questioned the long-term efficacy of these tests, and biomarkers that accurately identify PCa are needed.\r\nMETHODS:\r\nWe analysed the levels of circulating microRNAs (miRNAs) in patients with elevated PSA who were diagnosed with either localised PCa (n=36) or benign prostatic hyperplasia (BPH, n=31) upon biopsy. Real-time RT-PCR with Taqman probes was used to measure plasma levels of miRNAs. To circumvent problems associated with circulating miRNA quantitation, we computed the expression ratios of upregulated and downregulated miRNAs.\r\nRESULTS:\r\nThe miR-106a/miR-130b and miR-106a/miR-223 ratios were significantly different between the biopsy-positive and BPH groups (P<0.0001), and yielded statistical power values that were >0.99. Both miRNA ratios were highly sensitive and more specific than PSA in discriminating localised PCa from BPH. Receiver operating characteristic curve analysis revealed area under curve values of 0.81 (miR-106a/miR-130b) and 0.77 (miR-106a/miR-223).\r\nCONCLUSIONS:\r\nTesting for circulating miR-106a/miR-130b and miR-106a/miR-223 ratios may reduce the costs and morbidity of unnecessary biopsies and is feasible for large-scale screening, as it requires measuring only three miRNAs.
Lingua originaleInglese
pagine (da-a)1362-6-1366
RivistaBritish Journal of Cancer
Volume114
Numero di pubblicazione12
DOI
Stato di pubblicazionePubblicato - 2016

All Science Journal Classification (ASJC) codes

  • Oncologia
  • Ricerca sul Cancro

Keywords

  • prostate cancer screening

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