A 1 H NMR metabolomic approach for the estimation of the time since death using aqueous humour: an animal model

Fabio De Giorgio, Emanuela Locci, Matteo Stocchero, Antonio Noto, Alberto Chighine, Luca Natali, Pietro Emanuele Napoli, Roberto Caria, Matteo Nioi, Ernesto D’Aloja

Risultato della ricerca: Contributo in rivistaArticolo in rivista

7 Citazioni (Scopus)

Abstract

Introduction: The estimation of the time since death, or post-mortem interval (PMI), still remains a main conundrum in forensic science. Several approaches have been so far proposed from either a qualitative or a quantitative point of view, but they still lack reliability and robustness. Recently, metabolomics has shown to be a potential tool to investigate the time-related post-mortem metabolite modifications in animal models. Objectives: Here we propose, for the first time, the use of a 1 H NMR metabolomic approach for the estimation of PMI from aqueous humour (AH) in an ovine model. Methods: AH samples were collected at different times after death (from 118 to 1429 min). 1 H NMR experiments were performed and spectral data analysed by multivariate statistical tools. Results: A multivariate calibration model was built to estimate PMI on the basis of the metabolite content of the samples. The model was validated with an independent test set, obtaining a prediction error of 59 min for PMI < 500 min, 104 min for PMI from 500 to 1000 min, and 118 min for PMI > 1000 min. Moreover, the metabolomic approach suggested a picture of the mechanisms underlying the post-mortem biological modifications, highlighting the role played by taurine, choline, and succinate. Conclusion: The time-related modifications of the 1 H NMR AH metabolomic profile seem to be encouraging in addressing the issue of a reproducible and robust model to be employed for the estimation of the time since death.
Lingua originaleEnglish
pagine (da-a)N/A-N/A
RivistaMetabolomics
Volume15
DOI
Stato di pubblicazionePubblicato - 2019

Keywords

  • 1
  • Animal model
  • Animals
  • Aqueous Humor
  • Aqueous humour
  • Disease Models, Animal
  • Female
  • H NMR
  • Metabolomics
  • PMI estimation
  • Postmortem Changes
  • Proton Magnetic Resonance Spectroscopy
  • Sheep
  • Time Factors

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