TY - JOUR
T1 - 200+Protein Concentrations in Healthy Human Blood Plasma: Targeted
Quantitative SRM SIS Screening of Chromosomes 18, 13, Y, and the
Mitochondrial Chromosome Encoded Proteome
AU - Kopylov, Arthur T.
AU - Ponomarenko, Elena A.
AU - Ilgisonis, Ekaterina V.
AU - Pyatnitskiy, Mikhail A.
AU - Lisitsa, Andrey V.
AU - Poverennaya, Ekaterina V.
AU - Kiseleva, Olga I.
AU - Farafonova, Tatiana E.
AU - Tikhonova, Olga V.
AU - Zavialova, Maria G.
AU - Novikova, Svetlana E.
AU - Moshkovskii, Sergey A.
AU - Radko, Sergey P.
AU - Morukov, Boris V.
AU - Grigoriev, Anatoly I.
AU - Paik, Young-Ki
AU - Salekdeh, Ghasem Hosseini
AU - Urbani, Andrea
AU - Zgoda, Victor G.
AU - Archakov, Alexander I.
PY - 2019
Y1 - 2019
N2 - Mitochondria are undeniably the cell powerhouse,
directly affecting cell survival and fate. Growing
evidence suggest that mitochondrial protein repertoire affects
metabolic activity and plays an important role in determining
cell proliferation/differentiation or quiescence shift. Consequently,
the bioenergetic status of a cell is associated with
the quality and abundance of the mitochondrial populations
and proteomes. Mitochondrial morphology changes in the
development of different cellular functions associated with
metabolic switches. It is therefore reasonable to speculate that
different cell lines do contain different mitochondrialassociated
proteins, and the investigation of these pools may
well represent a source for mining missing proteins (MPs). A
very effective approach to increase the number of IDs through mass spectrometry consists of reducing the complexity of the
biological samples by fractionation. The present study aims at investigating the mitochondrial proteome of five phenotypically
different cell lines, possibly expressing some of the MPs, through an enrichment−fractionation approach at the organelle and
protein level. We demonstrate a substantial increase in the proteome coverage, which, in turn, increases the likelihood of
detecting low abundant proteins, often falling in the category of MPs, and resulting, for the present study, in the identification of
METTL12, FAM163A, and RGS13. All MS data have been deposited to the MassIVE data repository (https://massive.ucsd.
edu) with the data set identifier MSV000082409 and PXD010446.
AB - Mitochondria are undeniably the cell powerhouse,
directly affecting cell survival and fate. Growing
evidence suggest that mitochondrial protein repertoire affects
metabolic activity and plays an important role in determining
cell proliferation/differentiation or quiescence shift. Consequently,
the bioenergetic status of a cell is associated with
the quality and abundance of the mitochondrial populations
and proteomes. Mitochondrial morphology changes in the
development of different cellular functions associated with
metabolic switches. It is therefore reasonable to speculate that
different cell lines do contain different mitochondrialassociated
proteins, and the investigation of these pools may
well represent a source for mining missing proteins (MPs). A
very effective approach to increase the number of IDs through mass spectrometry consists of reducing the complexity of the
biological samples by fractionation. The present study aims at investigating the mitochondrial proteome of five phenotypically
different cell lines, possibly expressing some of the MPs, through an enrichment−fractionation approach at the organelle and
protein level. We demonstrate a substantial increase in the proteome coverage, which, in turn, increases the likelihood of
detecting low abundant proteins, often falling in the category of MPs, and resulting, for the present study, in the identification of
METTL12, FAM163A, and RGS13. All MS data have been deposited to the MassIVE data repository (https://massive.ucsd.
edu) with the data set identifier MSV000082409 and PXD010446.
KW - Chromosome-centric Human Proteome Project (C-HPP)
KW - Human Plasma Proteome Project (HPPP)
KW - healthy human
KW - plasma proteome
KW - selected reaction monitoring (SRMSIS)
KW - targeted proteomics
KW - Chromosome-centric Human Proteome Project (C-HPP)
KW - Human Plasma Proteome Project (HPPP)
KW - healthy human
KW - plasma proteome
KW - selected reaction monitoring (SRMSIS)
KW - targeted proteomics
UR - http://hdl.handle.net/10807/134503
U2 - 10.1021/acs.jproteome.8b00391
DO - 10.1021/acs.jproteome.8b00391
M3 - Article
SN - 1535-3907
VL - 18
SP - 120
EP - 129
JO - Journal of Proteome Research
JF - Journal of Proteome Research
ER -