1H NMR detects different metabolic profiles in glioblastoma stem-like cells

Laura Guidoni, Lucia Ricci-Vitiani, Antonella Rosi, Alessandra Palma, Sveva Grande, Anna Maria Luciani, Federica Pelacchi, Simona Di Martino, Cesare Colosimo, Mauro Biffoni, Ruggero De Maria Marchiano, Roberto Pallini, Vincenza Viti

Risultato della ricerca: Contributo in rivistaArticolo in rivista

18 Citazioni (Scopus)


The metabolic profiles of glioblastoma stem-like cells (GSCs) growing in neurospheres were examined by 1H NMR spectroscopy. Spectra of two GSC lines, labelled 1 and 83, from tumours close to the subventricular zone of the temporal lobe were studied in detail and compared with those of neural stem/progenitor cells from the adult olfactory bulb (OB-NPCs) and of the T98G glioblastoma cell line. In both GSCs, signals from myoinositol (Myo-I), UDP-hexosamines (UDP-Hex) and glycine indicated an astrocyte/glioma metabolism. For line 1, the presence of signals from N-acetyl aspartate, GABA and creatine pointed to a neuronal fingerprint. These metabolites were almost absent from line 83 spectra, whereas lipid signals, absent from normal neural lineages, were intense in line 83 spectra and remained low in those of line 1, irrespective of apoptotic fate. Spectra of OB-NPC cells displayed strong similarities with those from line 1, with low lipid signals and clearly detectable neuronal signals. In contrast, the spectral profile of line 83 was more similar to that of T98G, displaying high lipids and nearly complete absence of the neuronal markers. A mixed neural-astrocyte metabolic phenotype with a strong neuronal fingerprint was therefore found in line 1, while an astrocytic/glioma-like metabolism prevailed in line 83. We found a signal assigned to the amide proton of N-acetyl galactosamine in GSC lines and in OB-NPC spectra, whereas it was absent from those of T98G cells. This signal may be related to a stem-cell-specific protein glycosylation pattern and is therefore suggested as a marker of cell multipotency. Other GSC lines from patients with different clinical outcomes were then examined. Unsupervised analysis of spectral data from 13 lines yielded two clusters, with six lines resembling spectral features of line 1 and seven resembling those of line 83, suggesting that distinct metabolic phenotypes may be present in GSC lines. © 2013 John Wiley & Sons, Ltd.
Lingua originaleEnglish
pagine (da-a)129-145
Numero di pagine17
RivistaNMR in Biomedicine
Stato di pubblicazionePubblicato - 2014


  • 1H NMR spectroscopy
  • Biomarkers, Tumor
  • Brain Neoplasms
  • Cell Line, Tumor
  • Cell metabolism
  • GABA
  • Glioblastoma
  • Glioblastoma stem-like cells
  • Humans
  • Lipids
  • Magnetic Resonance Spectroscopy
  • Metabolic profiles
  • Molecular Medicine
  • N-acetyl aspartate
  • Neoplastic Stem Cells
  • Neural Stem Cells
  • Protons
  • Radiology, Nuclear Medicine and Imaging
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectroscopy


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