Supporting data for "Genome assembly and transcriptome resource for river buffalo, Bubalus bubalis (2n=50)"

  • John L. Williams (Creator)
  • Daniela Iamartino (Creator)
  • Kim D. Pruitt (Creator)
  • Tad Sonstegard (Creator)
  • Timothy P. L. Smith (Creator)
  • Wai Yee Low (Creator)
  • Tommaso Biagini (Creator)
  • Lorenzo Bomba (Creator)
  • Stefano Capomaccio (Creator)
  • Bianca Castiglioni (Creator)
  • Angelo Coletta (Creator)
  • Federica Corrado (Creator)
  • Fabrizio Ferrè (Contributor)
  • Leopoldo Iannuzzi (Creator)
  • Cynthia Lawley (Creator)
  • Nicolò Macciotta (Creator)
  • Matthew Mcclure (Creator)
  • Giordano Mancini (Creator)
  • Donato Matassino (Creator)
  • Raffaele Mazza (Creator)
  • Marco Milanesi (Creator)
  • Bianca Moioli (Creator)
  • Nicola Morandi (Creator)
  • Luigi Ramunno (Creator)
  • Vincenzo Peretti (Creator)
  • Fabio Pilla (Creator)
  • Paola Ramelli (Creator)
  • Steven Schroeder (Creator)
  • Francesco Strozzi (Creator)
  • Françoise Thibaud-Nissen (Contributor)
  • Luigi Zicarelli (Creator)
  • Paolo Ajmone Marsan (Creator)
  • Alessio Valentini (Creator)
  • Giovanni Chillemi (Creator)
  • Aleksey Zimin (Creator)

Dataset

Description

Water buffalo is a globally important species for agriculture and local economies. A de novo assembled, well annotated, reference sequence for the water buffalo is an important prerequisite for studying the biology of this species, and necessary to manage genetic diversity and to use modern breeding and genomic selection techniques. However, no such genome assembly has been previously reported. There are two species of domestic water buffalo, the river (2n=50) and the swamp (2n=48) buffalo. Here we describe a draft quality reference sequence for the river buffalo created from Illumina GA and Roche 454 short read sequences using the MaSuRCA assembler. The assembled sequence is 2.83 Gb, consisting of 366,983 scaffolds with a scaffold N50 of 1.41 Mb and contig N50 of 21,398 bp. Annotation of the genome was supported by transcriptome data from 30 tissues, and identified 21,711 predicted protein coding genes. Searches for complete mammalian BUSCO gene groups found 98.6% of curated single copy orthologs present among predicted genes, which suggests a high level of completeness of the genome. The annotated sequence is available from NCBI at accession GCA_000471725.1.
Dati resi disponibili18 ago 2017
EditoreGigaScience Database

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