Additional file 5: of G-quadruplex ligand RHPS4 radiosensitizes glioblastoma xenograft in vivo through a differential targeting of bulky differentiated- and stem-cancer cells

Figure S5. RHPS4 does not affect stem cell markers in U251MG-Sph and GSCs. Western blot analysis of stemness markers SOX2, NESTIN and CD44 in response to RHPS4 treatment in GSCs#1, #61, #83 and #163 (A) and U251MG-Adh and U251MG-Sph cells (B). Protein levels of CD44 (C), SOX2 (D) and NESTIN (E). Box plot represents mean, maximum and minimum values (n = 2). Gene expression profile for CD44 (F), SOX2 (G) and NESTIN (H). Box plot represents mean, maximum and minimum values (n = 2). (TIF 21948 kb)