Additional file 5 of An organoid model of colorectal circulating tumor cells with stem cell features, hybrid EMT state and distinctive therapy response profile

Additional file 5: Figure S1. MSI status/mutational profile of patient’s CRC and xenograft validation. (A) WES analysis was performed on the surgical sample used for organoid generation, allowing the estimation of mutation rates (hypermutated: more than 10 mutations per Megabase). High-grade microsatellite instability (MSI) was also detected. Both Non-Silent (blue) and Silent (orange) somatic variants are reported. (B) OncoPrint showing functionally relevant intragenic lesions in recurrently mutated genes. Half boxes and full boxes represent heterozygous and homozygous variants, respectively; colors are used to specify the type of mutation. Stars indicate germline mutations; multiple hits affecting the same gene are indicated by numbers. (C) Hematoxylin/Eosin staining of the primary patient’s tumor and of PDOs-derived subcutaneous tumor xenograft showing comparable histological structure. Magnification 20x.