Additional file 3: of G-quadruplex ligand RHPS4 radiosensitizes glioblastoma xenograft in vivo through a differential targeting of bulky differentiated- and stem-cancer cells

Figure S3. Telomere length analysis in cancer stem-like cells treated with RHPS4. Telomere lengths were analyzed using centromere-calibrated QFISH. Representative images of metaphase spreads from U251MG-Adh and -Sph cells (A). Box Plot of telomere lengths in untreated and RHPS4 treated U251MG-Adh, U251MG-Sph, GSCs#1, #61, #83 and #163. Box plot represents means and quartiles, whiskers represent s.d. and points represent outliers. (B) Percentage of telomeres shorter than 5 T/C% (red bars), comprised between 6 and 10 T/C% (green bars) and longer than 11 T/C% (blue bars) as evaluated in untreated and RHPS4 treated cells (C). (TIF 30408 kb)