Valutazione in vitro e in vivo dell’efficacia di analoghi del PBO contro popolazioni resistenti di insetti dannosi.

Translated title of the contribution: [Autom. eng. transl.] In vitro and in vivo evaluation of the efficacy of PBO analogues against resistant populations of harmful insects.

Emanuele Mazzoni, Michela Panini, Olga Chiesa, Valeria Todeschini, Vincenzo Puggioni, Matteo Anaclerio

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

[Autom. eng. transl.] The high use of synthetic insecticides for the control of harmful insects over the years has contributed to environmental pollution and has led to the establishment of resistant populations that render treatments ineffective. The problem of the appearance and diffusion of various mechanisms of resistance to various active principles has recently been aggravated by the withdrawal from the market of numerous products with different modes of action. Despite this, control by chemical means remains one of the main tools of phytosanitary practices and therefore it is important to find alternative approaches that can contribute to maintaining the effectiveness of the insecticides available today. A possible solution is represented by the combination of insecticides with synergistic molecules, such as the well-known piperonyl butoxide (PBO). Synergists are compounds that are non-toxic in themselves but able to increase the effectiveness of insecticides as they are capable of inhibiting the main detoxifying enzymes (esterases and monooxygenases) which are the main tools for defending insects. Starting from current knowledge on PBO, new analogues have been synthesized and evaluated in vitro for their ability to inhibit esterases and mono-oxygenases of different target insects, in order to clarify the possible interactions between enzymes and the chemical structures of the synergists developed. The compounds that showed a greater inhibitory capacity were selected and subsequently evaluated in vivo through laboratory bioassays on resistant populations, using commercial formulations of insecticides (pyrethroids and neonicotinoids). The data obtained showed the effective synergistic capacity of some analogues, with different performances depending on the species investigated, allowing a reduction in the dose of insecticide to be applied to reach the same mortality rates. The work was developed within the European project "Ecosyn" (EcoSyn -Ecofriendly synergists for insecticide formulations, funded by the Seventh Framework Program of the European Union - Contract no 605740) which aims to optimize the synthesis and use of specific molecules synergists in agriculture and in the anthropic environment, with consequent benefits for the environment (including pollinators) and for the health of operators and end consumers.
Translated title of the contribution[Autom. eng. transl.] In vitro and in vivo evaluation of the efficacy of PBO analogues against resistant populations of harmful insects.
Original languageItalian
Title of host publicationAtti XXV Congresso Nazionale Italiano di Entomologia
Pages275
Number of pages1
Publication statusPublished - 2016
EventXXV Congresso Nazionale Italiano di Entomologia - Padova
Duration: 20 Jun 201624 Jun 2016

Conference

ConferenceXXV Congresso Nazionale Italiano di Entomologia
CityPadova
Period20/6/1624/6/16

Keywords

  • EcoSyn
  • Myzus persicae
  • biosaggi
  • sinergici

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