Tracking the Biostimulatory Effect of Fractions from a Commercial Plant Protein Hydrolysate in Greenhouse-Grown Lettuce

Francesco Cristofano; Christophe El-Nakhel; Giuseppe Colla; Mariateresa Cardarelli; Youry Pii; Luigi Lucini; Youssef Rouphael

doi:10.3390/antiox12010107

Tracking the Biostimulatory Effect of Fractions from a Commercial Plant Protein Hydrolysate in Greenhouse-Grown Lettuce

Francesco Cristofano, Christophe El-Nakhel, Giuseppe Colla, Mariateresa Cardarelli, Youry Pii, Luigi Lucini, Youssef Rouphael

Research output: Contribution to journal › Article

Abstract

Protein hydrolysate biostimulants are environmentally friendly options for the reduction of nitrogen input, but their plant growth-promoting mechanisms are still not completely unveiled. Here, to put the “signaling peptide theory” to the test, a greenhouse experiment was undertaken using low (1 mM) and optimal (8 mM) NO3-treated butterhead lettuce and three molecular fractions (PH1 (>10 kDa), PH2 (1–10 kDa) and PH3 (<10 kDa) fractions), in addition to the whole product Vegamin®: PH, in a randomized block design. PH1 and PH3 significantly increased fresh yield (+8%) under optimal (lighter leaves), but not under low (darker leaves) NO3 conditions. Total ascorbic acid, lutein and β-carotene increased with PH3, and disinapoylgentobiose and kaempferol-3-hydroxyferuloyl-sophorosie-7-glucoside content increased with PH (whole/fractions) treatments, particularly under low NO3 conditions. The complete hydrolysate and analyzed peptide fractions have differential biostimulatory effects, enhancing the growth and nutritional quality of lettuce.

Original language	English
Pages (from-to)	N/A-N/A
Journal	Antioxidants
Volume	12
DOIs	https://doi.org/10.3390/antiox12010107
Publication status	Published - 2023

Keywords

Orbitrap LC-MS/MS
UHPLC
ascorbic acid
chlorogenic acid
fresh weight
low nitrogen
peptides
polyphenolics
produce quality
secondary metabolism

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10.3390/antiox12010107

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title = "Tracking the Biostimulatory Effect of Fractions from a Commercial Plant Protein Hydrolysate in Greenhouse-Grown Lettuce",

abstract = "Protein hydrolysate biostimulants are environmentally friendly options for the reduction of nitrogen input, but their plant growth-promoting mechanisms are still not completely unveiled. Here, to put the “signaling peptide theory” to the test, a greenhouse experiment was undertaken using low (1 mM) and optimal (8 mM) NO3-treated butterhead lettuce and three molecular fractions (PH1 (>10 kDa), PH2 (1–10 kDa) and PH3 (<10 kDa) fractions), in addition to the whole product Vegamin{\textregistered}: PH, in a randomized block design. PH1 and PH3 significantly increased fresh yield (+8%) under optimal (lighter leaves), but not under low (darker leaves) NO3 conditions. Total ascorbic acid, lutein and β-carotene increased with PH3, and disinapoylgentobiose and kaempferol-3-hydroxyferuloyl-sophorosie-7-glucoside content increased with PH (whole/fractions) treatments, particularly under low NO3 conditions. The complete hydrolysate and analyzed peptide fractions have differential biostimulatory effects, enhancing the growth and nutritional quality of lettuce.",

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author = "Francesco Cristofano and Christophe El-Nakhel and Giuseppe Colla and Mariateresa Cardarelli and Youry Pii and Luigi Lucini and Youssef Rouphael",

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T1 - Tracking the Biostimulatory Effect of Fractions from a Commercial Plant Protein Hydrolysate in Greenhouse-Grown Lettuce

AU - Cristofano, Francesco

AU - El-Nakhel, Christophe

AU - Colla, Giuseppe

AU - Cardarelli, Mariateresa

AU - Pii, Youry

AU - Lucini, Luigi

AU - Rouphael, Youssef

PY - 2023

Y1 - 2023

N2 - Protein hydrolysate biostimulants are environmentally friendly options for the reduction of nitrogen input, but their plant growth-promoting mechanisms are still not completely unveiled. Here, to put the “signaling peptide theory” to the test, a greenhouse experiment was undertaken using low (1 mM) and optimal (8 mM) NO3-treated butterhead lettuce and three molecular fractions (PH1 (>10 kDa), PH2 (1–10 kDa) and PH3 (<10 kDa) fractions), in addition to the whole product Vegamin®: PH, in a randomized block design. PH1 and PH3 significantly increased fresh yield (+8%) under optimal (lighter leaves), but not under low (darker leaves) NO3 conditions. Total ascorbic acid, lutein and β-carotene increased with PH3, and disinapoylgentobiose and kaempferol-3-hydroxyferuloyl-sophorosie-7-glucoside content increased with PH (whole/fractions) treatments, particularly under low NO3 conditions. The complete hydrolysate and analyzed peptide fractions have differential biostimulatory effects, enhancing the growth and nutritional quality of lettuce.

AB - Protein hydrolysate biostimulants are environmentally friendly options for the reduction of nitrogen input, but their plant growth-promoting mechanisms are still not completely unveiled. Here, to put the “signaling peptide theory” to the test, a greenhouse experiment was undertaken using low (1 mM) and optimal (8 mM) NO3-treated butterhead lettuce and three molecular fractions (PH1 (>10 kDa), PH2 (1–10 kDa) and PH3 (<10 kDa) fractions), in addition to the whole product Vegamin®: PH, in a randomized block design. PH1 and PH3 significantly increased fresh yield (+8%) under optimal (lighter leaves), but not under low (darker leaves) NO3 conditions. Total ascorbic acid, lutein and β-carotene increased with PH3, and disinapoylgentobiose and kaempferol-3-hydroxyferuloyl-sophorosie-7-glucoside content increased with PH (whole/fractions) treatments, particularly under low NO3 conditions. The complete hydrolysate and analyzed peptide fractions have differential biostimulatory effects, enhancing the growth and nutritional quality of lettuce.

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KW - UHPLC

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KW - chlorogenic acid

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KW - low nitrogen

KW - peptides

KW - polyphenolics

KW - produce quality

KW - secondary metabolism

KW - Orbitrap LC-MS/MS

KW - UHPLC

KW - ascorbic acid

KW - chlorogenic acid

KW - fresh weight

KW - low nitrogen

KW - peptides

KW - polyphenolics

KW - produce quality

KW - secondary metabolism

UR - http://hdl.handle.net/10807/232262

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SP - N/A-N/A

JO - Antioxidants

JF - Antioxidants

ER -

Tracking the Biostimulatory Effect of Fractions from a Commercial Plant Protein Hydrolysate in Greenhouse-Grown Lettuce

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Keywords

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