Top-down proteomic characterization of DAOY medulloblastoma tumor cell line

Federica Iavarone, Gianpiero Tamburrini, Wanda Lattanzi, Claudia Martelli, Luca D'Angelo, Marta Barba, Mirko Baranzini, Ilaria Inserra, Federica Vincenzoni, Luca Massimi, Concezio Di Rocco, Massimo Caldarelli, Irene Messana, Fabrizio Michetti, Massimo Castagnola, Claudia Desiderio

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The proteome of the DAOY medulloblastoma cell line has been investigated by an LC-MS top-down platform. This approach, unlike the bottom-up one, allows identifying proteins and peptides in their intact forms, and disclosing post-translational modifications, proteoforms and naturally occurring peptides. Indeed, 25 out of the 53 proteins identified, were not previously characterized in DAOY cells. Most of them were functionally interconnected, being mainly involved in binding, catalytic and structural activities, and metabolic processes. The top-down approach, applied in this preliminary study, disclosed the presence of several naturally occurring peptide fragments that strongly characterized DAOY cell intact proteome.
Original languageEnglish
Pages (from-to)13-21
Number of pages9
JournalEuPA Open Proteomics
Volume12
DOIs
Publication statusPublished - 2016

Keywords

  • Biochemistry
  • DAOY cell line
  • Medulloblastoma
  • Peptidome
  • Proteome
  • Top-down proteomics

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