Caveolin-1 (cav-1) is the principal structural component of caveolae which functions as scaffoldiprotein for the integration of a variety of signaling pathways. In this study, we show that siRNA-induced cav-1 down regulation in human endothelial cells (EC) increased cell size and provoked cell cycle arrest at G1/S phase transition. In addition, silencing of cav-1 reduced matrix metalloproteinases (MMPs) activity which, in turn, affected cell migration and VEGF-induced tube formation of EC in vitro. These data indicate that proper expression of cav-1 is required for maintaining typical functions of EC such as proliferation and the formation of new blood vessels. In addition, we observed a marked increase of cell size, after cav-1 silencing, which might indicate the involvement of this scaffolding protein in the way by which cells perceive changes in their microenvironment. In conclusion, this study proposes cav-1 as an interesting target molecule for studying cellular mechanisms which occur in physiological as well as pathological conditions such as senescence and tumorigenesis.
|Title of host publication||ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY|
|Number of pages||1|
|Publication status||Published - 2012|
|Event||CONGRESSO NAZIONALE DELLA SOCIETA' ITALIANA DI ANATOMIA E ISTOLOGIA (SIAI) - Pistoia|
Duration: 20 Sep 2012 → 23 Sep 2012
|Conference||CONGRESSO NAZIONALE DELLA SOCIETA' ITALIANA DI ANATOMIA E ISTOLOGIA (SIAI)|
|Period||20/9/12 → 23/9/12|