The Enterococcus faecalis virulence factor ElrA interacts with the human Four-And-A-Half LIM Domains Protein

Alexandre Jamet, Rozenn Dervyn, Nicolas Lapaque, Francesca Bugli, Naima G. Perez-Cortez, Hervé M. Blottière, Jean-Claude Twizere, Maurizio Sanguinetti, Brunella Posteraro, Pascale Serror, Emmanuelle Maguin

Research output: Contribution to journalArticle

3 Citations (Scopus)


The commensal bacterium Enterococcus faecalis is a common cause of nosocomial infections worldwide. The increasing prevalence of multi-Antibiotic resistant E. faecalis strains reinforces this public health concern. Despite numerous studies highlighting several pathology-related genetic traits, the molecular mechanisms of E. faecalis virulence remain poorly understood. In this work, we studied 23 bacterial proteins that could be considered as virulence factors or involved in the Enterococcus interaction with the host. We systematically tested their interactions with human proteins using the Human ORFeome library, a set of 12,212 human ORFs, in yeast. Among the thousands of tested interactions, one involving the E. faecalis virulence factor ElrA and the human protein FHL2 was evidenced by yeast two-hybrid and biochemically confirmed. Further molecular characterizations allowed defining an FHL2-interacting domain (FID) of ElrA. Deletion of the FID led to an attenuated in vivo phenotype of the mutated strain clearly indicating that this interaction is likely to contribute to the multifactorial virulence of this opportunistic pathogen. Altogether, our results show that FHL2 is the first host cellular protein directly targeted by an E. faecalis virulence factor and that this interaction is involved in Enterococcus pathogenicity.
Original languageEnglish
Pages (from-to)4581-N/A
JournalScientific Reports
Publication statusPublished - 2017


  • Enterococcus


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