The combination of whole cell lipidomics analysis and single cell confocal imaging of fluidity and micropolarity provides insight into stress-induced lipid turnover in subcellular organelles of pancreatic beta cells

Giuseppe Maulucci, Ofir Cohen, Bareket Daniel, Carla Ferreri, Shlomo Sasson

Research output: Contribution to journalArticlepeer-review

Abstract

Modern omics techniques reveal molecular structures and cellular networks of tissues and cells in unprecedented detail. Recent advances in single cell analysis have further revolutionized all disciplines in cellular and molecular biology. These methods have also been employed in current investigations on the structure and function of insulin secreting beta cells under normal and pathological conditions that lead to an impaired glucose tolerance and type 2 diabetes. Proteomic and transcriptomic analyses have pointed to significant alterations in protein expression and function in beta cells exposed to diabetes like conditions (e.g., high glucose and/or saturated fatty acids levels). These nutritional overload stressful conditions are often defined as glucolipotoxic due to the progressive damage they cause to the cells. Our recent studies on the rat insulinoma-derived INS-1E beta cell line point to differential effects of such conditions in the phospholipid bilayers in beta cells. This review focuses on confocal microscopy-based detection of these profound alterations in the plasma membrane and membranes of insulin granules and lipid droplets in single beta cells under such nutritional load conditions.
Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalMolecules
Volume24
DOIs
Publication statusPublished - 2019

Keywords

  • Beta cells
  • Cell micropolarity maps
  • Confocal microscopy
  • Diabetes
  • Lipidomics
  • Membrane fluidity maps

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