Tetanus neurotoxin-induced epilepsy in mouse visual cortex

Marco Mainardi, Marta Pietrasanta, Eleonora Vannini, Ornella Rossetto, Matteo Caleo

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Tetanus neurotoxin (TeNT) is a metalloprotease that cleaves the synaptic protein VAMP/synaptobrevin, leading to focal epilepsy. Although this model is widely used in rats, the time course and spatial specificity of TeNT proteolytic action have not been precisely defined. Here we have studied the biochemical, electrographic, and anatomic characteristics of TeNT-induced epilepsy in mouse visual cortex (V1). We found that VAMP cleavage peaked at 10 days, was reduced at 21 days, and completely extinguished 45 days following TeNT delivery. VAMP proteolysis was restricted to the injected V1 and ipsilateral thalamus, whereas it was undetectable in other cortical areas. Electrographic epileptiform activity was evident both during and after the time window of TeNT effects, indicating development of chronic epilepsy. Anatomic analyses found no evidence for long-term tissue damage, such as neuronal loss or microglia activation. These data show that TeNT reliably induces nonlesional epilepsy in mouse cortex. Due to the excellent physiologic knowledge of the visual cortex and the availability of mouse transgenic strains, this model will be useful for examining the network and cellular alterations underlying hyperexcitability within an epileptic focus.
Original languageEnglish
Pages (from-to)e132-e136
Number of pages5
Publication statusPublished - 2012


  • Action Potentials
  • Animals
  • Calcium-Binding Proteins
  • Disease Models, Animal
  • Epilepsy
  • Gene Expression Regulation
  • Mice
  • Mice, Inbred C57BL
  • Neurotoxins
  • Tetanus Toxin
  • Time Factors
  • Vesicle-Associated Membrane Protein 2
  • Visual Cortex


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