Technical note: Capillary electrophoresis as a rapid test for the quantification of immunoglobulin G in serum of newborn lambs

Vincenzo Lopreiato, Andrea Minuti, Erminio Trevisi, V. M. Morittu, C. Ceniti, A. A. Spina, D. Britti, F. Trimboli

Research output: Contribution to journalArticlepeer-review

Abstract

Finding a rapid and simple method of serum IgG determination in lambs is essential for monitoring failure of passive transfer of immunity. The aim of this study was to assess the ability of capillary electrophoresis (CE), an instrument mainly used in blood serum protein analysis, to estimate IgG content in serum of newborn lambs through determination of only total Ig percentage by comparing the results with those obtained with radial immunodiffusion (RID), the reference method for serum IgG quantification. Serum samples were collected at 24 h after birth from 40 Sarda lambs. The IgG concentration measured by RID and serum total Ig concentration measured by CE were (mean ± standard deviation) 29.8 ± 16.1 g/L and 37.8 ± 15.0%, respectively. Data provided by RID and CE analysis showed a polynomial trend (RID = 0.02CE2 − 0.04CE + 4.13; coefficient of determination, R2 = 0.96), displaying a strong relationship between these 2 methods. Applying the polynomial equation, the IgG values were predicted. Predicted IgG values were highly correlated (r = 0.98) and related (R2 = 0.96) to IgG values obtained by RID assay. These data were subjected to Bland–Altman analysis, revealing a high level of agreement between CE and RID methods with a bias that was not different from 0 (−0.04 g/L) and agreement limits of −6.38 g/L (low) and +6.30 g/L (high). In addition, the linear regression analysis between differences (dependent variable) and average of IgG concentration by CE and RID (independent variable) did not show proportional bias (R2 = 0.01). In conclusion, CE is a reliable instrument for a lamb health monitoring program, where Bland–Altman analysis also confirmed that the CE method can be a suitable alternative to RID.
Original languageEnglish
Pages (from-to)6583-6587
Number of pages5
JournalJournal of Dairy Science
Volume103
DOIs
Publication statusPublished - 2020

Keywords

  • Animals
  • Animals, Newborn
  • Electrophoresis, Capillary
  • Female
  • Immunodiffusion
  • Immunoglobulin G
  • Models, Statistical
  • Regression Analysis
  • Sheep
  • capillary electrophoresis
  • immunoglobulin
  • lamb
  • method comparison

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