Systemic endotoxin administration results in increased S100B protein blood levels and periventricular brain white matter injury in the preterm fetal sheep.

Yves Garnier, Richard Berger, Stephanie Alm, Monika U Von Duering, Audrey Bc Coumans, Fabrizio Michetti, Matteo Bruschettini, Mario Lituania, Tom Hm Hasaart, Diego Gazzolo

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

OBJECTIVE: Intrauterine infection is suggested to cause perinatal brain white matter injury. The aim of the present study was to clarify, whether intravenous application of endotoxin results in neuropathological findings and increased blood levels of the S100B protein, which is a consolidated marker of brain injury. METHODS: Twenty-one fetal sheep were chronically catheterized at a mean gestational age of 107+/-1 days (0.7 of gestation). Three days after surgery fetuses received either 100 (n=9), 500 (n=5) or 2500ng (n=1) lipopolysaccharide (LPS; E. coli; O127:B8, Sigma-Aldrich) or 2ml 0.9% saline (n=6) i.v. S100B protein blood levels were assessed before during and after LPS or placebo administration. Brain damage was evaluated by light microscopy. Selected areas of the periventricular white matter were also examined by electron microscopy. RESULTS: Histopathological screening revealed no evidence for cortical neuronal cell damage in both groups. However, LPS treatment resulted in inflammatory infiltrates in all animals and cystic lesions in the periventricular brain white matter in two fetuses. On electron micrographs, infiltrate forming cells appeared to be activated microglia. S100B protein blood levels were significantly higher in the LPS group at 1h (p<0.01) after LPS injection, peaking at 3h (p<0.001) and returning to baseline between 12 and 72h. CONCLUSION: Intravenous application of endotoxin caused focal periventricular brain white matter injury, inflammation and an increase in S100B protein release. It is suggested that longitudinal investigations of S100B protein blood levels offer a tool for the early detection of white matter injury.
Original languageEnglish
Pages (from-to)15-22
Number of pages8
JournalEUROPEAN JOURNAL OF OBSTETRICS, GYNECOLOGY, AND REPRODUCTIVE BIOLOGY
Publication statusPublished - 2006
Externally publishedYes

Keywords

  • BRAIN INJURY
  • CIRCULATION
  • ENDOTOXIN
  • ESCHERICHIA COLI
  • FETAL INFLAMMATORY RESPONSE SYNDROM
  • LIPOPOLYSACCARIDE
  • PERIVENTRICULAR LEUKOMALACIA

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