SINGLE CELL OBSERVATIONS OF GAS REACTIONS AND SHAPE CHANGES IN NORMAL AND SICKLING ERYTHROCYTES

Bruno Giardina, E Antonini, M Brunori, P Benedetti, G Bianchini, S. Grassi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Microspectrophotometry has been applied to single red blood cells to reinvestigate the linked processes of diffusion of gases inside the erythrocyte and their combination with hemoglobin. The experiments took advantage of the photosensitivity of the cabron monoxide derivative of hemoglobin, which allows ligand release from the CO-saturated red cells under strong illumination and recombination when the light is switched off. The photochemical method was also used to study the kinetics of sickling on ligand removal in single erythrocytes of Hb S carriers. The results give new information on the mechanism of the sickling process
Original languageEnglish
Pages (from-to)187-195
Number of pages9
JournalBiophysical Journal
Volume24
Publication statusPublished - 1978

Keywords

  • ERYTHROCYTES

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