Simultaneous detection of cocaine and heroin metabolites in urine by solid-phase extraction and gas chromatography-mass spectrometry

Nadia De Giovanni; Sabina Strano Rossi

doi:10.1016/0378-4347(94)00209-6

Simultaneous detection of cocaine and heroin metabolites in urine by solid-phase extraction and gas chromatography-mass spectrometry

Nadia De Giovanni, Sabina Strano Rossi

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The present paper reports a method for the simultaneous extraction of cocaine, heroin and their metabolites from small amounts of urine (0.5 ml), using deuterated internal standards. Solid-phase extraction (SPE) on Cls columns followed by chromatographic separation coupled with mass spectrometry allowed the detection of all the substances after their derivatization. Mass spectrometry was performed in the electron-impact selected-ion monitoring (EI-SIM) mode. The limit of detection was found to be as low as 50 ng/ml for all the analytes; for reproducibility the C.V. was always better than 7%; the method was found to be linear with correlation coefficients between 0.989 and 1.00

Original language	English
Pages (from-to)	69-73
Number of pages	5
Journal	JOURNAL OF CHROMATOGRAPHY B. BIOMEDICAL APPLICATIONS
Volume	658
DOIs	https://doi.org/10.1016/0378-4347(94)00209-6
Publication status	Published - 1994

Keywords

GC/MS
cocaine
forensic toxicology
heroin metabolites

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10.1016/0378-4347(94)00209-6

Cite this

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title = "Simultaneous detection of cocaine and heroin metabolites in urine by solid-phase extraction and gas chromatography-mass spectrometry",

abstract = "The present paper reports a method for the simultaneous extraction of cocaine, heroin and their metabolites from small amounts of urine (0.5 ml), using deuterated internal standards. Solid-phase extraction (SPE) on Cls columns followed by chromatographic separation coupled with mass spectrometry allowed the detection of all the substances after their derivatization. Mass spectrometry was performed in the electron-impact selected-ion monitoring (EI-SIM) mode. The limit of detection was found to be as low as 50 ng/ml for all the analytes; for reproducibility the C.V. was always better than 7%; the method was found to be linear with correlation coefficients between 0.989 and 1.00",

keywords = "GC/MS, cocaine, forensic toxicology, heroin metabolites, GC/MS, cocaine, forensic toxicology, heroin metabolites",

author = "{De Giovanni}, Nadia and {Strano Rossi}, Sabina",

year = "1994",

doi = "10.1016/0378-4347(94)00209-6",

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publisher = "Elsevier BV:PO Box 211, 1000 AE Amsterdam Netherlands:011 31 20 4853757, 011 31 20 4853642, 011 31 20 4853641, EMAIL: nlinfo-f@elsevier.nl, INTERNET: http://www.elsevier.nl, Fax: 011 31 20 4853598",

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TY - JOUR

T1 - Simultaneous detection of cocaine and heroin metabolites in urine by solid-phase extraction and gas chromatography-mass spectrometry

AU - De Giovanni, Nadia

AU - Strano Rossi, Sabina

PY - 1994

Y1 - 1994

N2 - The present paper reports a method for the simultaneous extraction of cocaine, heroin and their metabolites from small amounts of urine (0.5 ml), using deuterated internal standards. Solid-phase extraction (SPE) on Cls columns followed by chromatographic separation coupled with mass spectrometry allowed the detection of all the substances after their derivatization. Mass spectrometry was performed in the electron-impact selected-ion monitoring (EI-SIM) mode. The limit of detection was found to be as low as 50 ng/ml for all the analytes; for reproducibility the C.V. was always better than 7%; the method was found to be linear with correlation coefficients between 0.989 and 1.00

AB - The present paper reports a method for the simultaneous extraction of cocaine, heroin and their metabolites from small amounts of urine (0.5 ml), using deuterated internal standards. Solid-phase extraction (SPE) on Cls columns followed by chromatographic separation coupled with mass spectrometry allowed the detection of all the substances after their derivatization. Mass spectrometry was performed in the electron-impact selected-ion monitoring (EI-SIM) mode. The limit of detection was found to be as low as 50 ng/ml for all the analytes; for reproducibility the C.V. was always better than 7%; the method was found to be linear with correlation coefficients between 0.989 and 1.00

KW - GC/MS

KW - cocaine

KW - forensic toxicology

KW - heroin metabolites

KW - GC/MS

KW - cocaine

KW - forensic toxicology

KW - heroin metabolites

UR - http://hdl.handle.net/10807/28648

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U2 - 10.1016/0378-4347(94)00209-6

DO - 10.1016/0378-4347(94)00209-6

M3 - Article

SN - 0378-4347

VL - 658

SP - 69

EP - 73

JO - JOURNAL OF CHROMATOGRAPHY B. BIOMEDICAL APPLICATIONS

JF - JOURNAL OF CHROMATOGRAPHY B. BIOMEDICAL APPLICATIONS

ER -

Simultaneous detection of cocaine and heroin metabolites in urine by solid-phase extraction and gas chromatography-mass spectrometry

Abstract

Keywords

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