Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay

Tiziana D'Inzeo, Giulia De Angelis, Maurizio Sanguinetti, Brunella Posteraro, Teresa Spanu, Liliana Giordano, Barbara Fiori Alfarano, Flora Marzia Liotti, Giulia Menchinelli, Flavio De Maio, Gabriella Parisi, Francesco Luzzaro

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

We directly tested 484 organisms from clinical (n = 310) and simulated (n = 174) positive blood cultures using the NG-Test Carba 5 assay for carbapenemase-producing Enterobacterales detection. The assay identified all but 4 of the KPC (170/171), OXA-48-like (22/22), VIM (19/21), and NDM (14/15) producers with no false positives. Among the clinical Klebsiella pneumoniae organisms tested, 122 of 123 KPC, 1 of 1 OXA-48-like, and 1 of 2 VIM producers were detected by the assay. Some VIM and NDM producers yielded scant but still-readable bands with the assay. No organisms produced the IMPS that the assay was designed to detect.
Original languageEnglish
Pages (from-to)N/A-N/A
JournalAntimicrobial Agents and Chemotherapy
Volume63
DOIs
Publication statusPublished - 2019

Keywords

  • Carbapenem resistance
  • Enterobacterales
  • Immunochromatographic assay
  • Positive blood culture

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