Sevoflurane- and desflurane-induced human myocardial post-conditioning through Phosphatidylinositol-3-kinase/Akt signalling

Massimo Massetti, L Zhu, S Lemoine, G Babatasi, O Lepage, J. L Gérard, J. L. Hanouz

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

BACKGROUND: The role of phosphatidylinositol-3-kinase (PI3K) in sevoflurane- and desflurane-induced myocardial post-conditioning remains unknown. METHODS: We recorded isometric contraction of isolated human right atrial trabeculae (oxygenated Tyrode's at 34 degrees C, stimulation frequency 1 Hz). In all groups, a 30-min hypoxic period was followed by a 60-min reoxygenation period. At the onset of reoxygenation, muscles were exposed to 5 min of sevoflurane 1%, 2%, and 3%, and desflurane 3%, 6%, and 9%. In separate groups, sevoflurane 2% and desflurane 6% were administered in the presence of 100 nM wortmannin, a PI3K inhibitor. Recovery of force after the 60-min reoxygenation period was compared between groups (mean +/- SD). RESULT: As compared with the Control group (49 +/- 7% of baseline) PostC by sevoflurane 1%, 2%, and 3% (78 +/- 4%, 79 +/- 5%, and 85 +/- 4% of baseline, respectively) and desflurane 3%, 6%, and 9% (74 +/- 5%, 84 +/- 4%, and 86 +/- 11% of baseline, respectively) enhanced the recovery of force. This effect was abolished in the presence of wortmannin (56 +/- 5% of baseline for sevoflurane 2%+wortmannin; 56 +/- 3% of baseline for desflurane 6%+wortmannin). Wortmannin alone had no effect on the recovery of force (57 +/- 7% of baseline). CONCLUSION: In vitro, sevoflurane and desflurane post-conditioned human myocardium against hypoxia through activation of phosphatidylinositol-3-kinase.
Original languageEnglish
Pages (from-to)949-956
Number of pages8
JournalActa Anaesthesiologica Scandinavica
Volume53
DOIs
Publication statusPublished - 2009

Keywords

  • Aged
  • Androstadienes
  • Anesthetics, Inhalation
  • Enzyme Activation
  • Enzyme Inhibitors
  • Female
  • Heart
  • Heart Atria
  • Humans
  • Hypoxia
  • In Vitro Techniques
  • Ischemic Preconditioning, Myocardial
  • Isoflurane
  • Male
  • Methyl Ethers
  • Middle Aged
  • Myocardium
  • Oncogene Protein v-akt
  • Organ Size
  • Phosphatidylinositol 3-Kinases
  • Preanesthetic Medication
  • Signal Transduction

Fingerprint Dive into the research topics of 'Sevoflurane- and desflurane-induced human myocardial post-conditioning through Phosphatidylinositol-3-kinase/Akt signalling'. Together they form a unique fingerprint.

Cite this