SEPARATION OF REDUCED AND OXIDIZED GLUTATHIONE BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

The separation of reduced and oxidized glutathione at an absolute\r\nsensitivity of about 100 pg by micellar electrokinetic capillary chromatography without derivatization is described. The time required for the separation is less than 10 min (the time between two following injections is about 15 min). The separation is characterized by high\r\nefficiency and good reliability. A partition mechanism is responsible\r\nfor the high resolution observed. The method was utilized for the\r\nanalysis of commercial preparations of glutathione and a good agreement\r\nwith the expected results was obtained; the oxidation of the commercial\r\nglutathione in solution was easily analysed.