Separation of diadenosine polyphosphates by capillary electrophoresis.

Massimo Castagnola, Rosaria Medda, Alessandra Padiglia, Irene Messana, Giovanni Floris

Research output: Contribution to journalArticle

Abstract

The influence of buffer composition and pH on the electrophoretic behavior of diadenosine polyphosphates with a phosphate chain ranging from two to five phosphate groups has been examined. The electrophoretic mobility in carbonate buffer increases according to the number of phosphates, whereas in berate buffer the mobility changes in an irregular way as a function of pH. This finding can be rationalized by a well-known interaction of berate with ribose rings, which modifies the charge and the hydrodynamic radius of each diadenosine polyphosphate in a different way. Our study shows that the best separation of diadenosine polyphosphates can be achieved at the highest pH values of the range examined both in berate and carbonate buffers.
Original languageEnglish
Pages (from-to)2412-2416
Number of pages5
JournalElectrophoresis
Publication statusPublished - 2000

Keywords

  • diadenosine polifosfato
  • elettroforesi
  • elettroforesi capillare

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