Raw cow milk bacterial consortium as bioindicator of circulating anti-microbial resistance (Amr)

Viviana Greco, Andrea Urbani, Cristian Piras, Enrico Gugliandolo, Alessio Soggiu, Bruno Tilocca, Luigi Bonizzi, Alfonso Zecconi, Rainer Cramer, Domenico Britti, Paola Roncada

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The environment, including animals and animal products, is colonized by bacterial species that are typical and specific of every different ecological niche. Natural and human-related ecological pressure promotes the selection and expression of genes related to antimicrobial resistance (AMR). These genes might be present in a bacterial consortium but might not necessarily be expressed. Their expression could be induced by the presence of antimicrobial compounds that could originate from a given ecological niche or from human activity. In this work, we applied (meta)proteomics analysis of bacterial compartment of raw milk in order to obtain a method that provides a measurement of circulating AMR involved proteins and gathers information about the whole bacterial composition. Results from milk analysis revealed the presence of 29 proteins/proteoforms linked to AMR. The detection of mainly β-lactamases suggests the possibility of using the milk microbiome as a bioindicator for the investigation of AMR. Moreover, it was possible to achieve a culture-free qualitative and functional analysis of raw milk bacterial consortia.
Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalAnimals
Volume10
DOIs
Publication statusPublished - 2020

Keywords

  • AMR
  • Microbiome
  • Proteomics
  • Raw milk
  • β-lactamase

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