Putative Biomarkers for Malignant Pleural Mesothelioma Suggested by Proteomic Analysis of Cell Secretome.

Serena Lacerenza; Federica Ciregia; Laura Giusti; Alessandra Bonotti; Viviana Greco; Gino Giannaccini; Vanessa D’Antongiovanni; Poupak Fallahi; Luisa Pieroni; Alfonso Cristaudo; Antonio Lucacchini; Maria Rosa Mazzoni; Rudy Foddis

doi:10.21873/cgp.20183

Putative Biomarkers for Malignant Pleural Mesothelioma Suggested by Proteomic Analysis of Cell Secretome.

Serena Lacerenza
, Federica Ciregia
, Laura Giusti
, Alessandra Bonotti
, Viviana Greco
, Gino Giannaccini
, Vanessa D’Antongiovanni
, Poupak Fallahi
, Luisa Pieroni
, Alfonso Cristaudo
, Antonio Lucacchini
, Maria Rosa Mazzoni
, Rudy Foddis

University of Pisa
University of Camerino
IRCCS Fondazione Santa Lucia - Roma

Research output: Contribution to journal › Article

1 Citation (Scopus)

Abstract

BACKGROUND:Malignant pleural mesothelioma (MPM) a rare neoplasm linked to asbestos exposure is characterized by a poor prognosis. Soluble mesothelin is currently considered the most specific diagnostic biomarker. The aim of the study was to identify novel biomarkers by proteomic analysis of two MPM cell lines secretome. MATERIALS AND METHODS:The protein patterns of MPM cells secretome were examined and compared to a non-malignant mesothelial cell line using two-dimensional gel electrophoresis coupled to mass spectrometry. Serum levels of candidate biomarkers were determined in MPM patients and control subjects. RESULTS:Two up-regulated proteins involved in cancer biology, prosaposin and quiescin Q6 sulfhydryl oxidase 1, were considered candidate biomarkers. Serum levels of both proteins were significantly higher in MPM patients than control subjects. Combining the data of each receiver-operating characteristic analysis predicted a good diagnostic accuracy. CONCLUSION:A panel of the putative biomarkers represents a promising tool for MPM diagnosis.

Original language	English
Pages (from-to)	225-236
Number of pages	12
Journal	CANCER GENOMICS & PROTEOMICS.
DOIs	https://doi.org/10.21873/cgp.20183
Publication status	Published - 2020

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Malignant pleural mesothelioma
proteomic analysis

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10.21873/cgp.20183

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Lacerenza, S., Ciregia, F., Giusti, L., Bonotti, A., Greco, V., Giannaccini, G., D’Antongiovanni, V., Fallahi, P., Pieroni, L., Cristaudo, A., Lucacchini, A., Mazzoni, M. R., & Foddis, R. (2020). Putative Biomarkers for Malignant Pleural Mesothelioma Suggested by Proteomic Analysis of Cell Secretome. CANCER GENOMICS & PROTEOMICS., 225-236. https://doi.org/10.21873/cgp.20183

@article{a86c4ae2ac504e1fb75cf31c56cab946,

title = "Putative Biomarkers for Malignant Pleural Mesothelioma Suggested by Proteomic Analysis of Cell Secretome.",

abstract = "BACKGROUND:Malignant pleural mesothelioma (MPM) a rare neoplasm linked to asbestos exposure is characterized by a poor prognosis. Soluble mesothelin is currently considered the most specific diagnostic biomarker. The aim of the study was to identify novel biomarkers by proteomic analysis of two MPM cell lines secretome. MATERIALS AND METHODS:The protein patterns of MPM cells secretome were examined and compared to a non-malignant mesothelial cell line using two-dimensional gel electrophoresis coupled to mass spectrometry. Serum levels of candidate biomarkers were determined in MPM patients and control subjects. RESULTS:Two up-regulated proteins involved in cancer biology, prosaposin and quiescin Q6 sulfhydryl oxidase 1, were considered candidate biomarkers. Serum levels of both proteins were significantly higher in MPM patients than control subjects. Combining the data of each receiver-operating characteristic analysis predicted a good diagnostic accuracy. CONCLUSION:A panel of the putative biomarkers represents a promising tool for MPM diagnosis.",

keywords = "Malignant pleural mesothelioma, proteomic analysis, Malignant pleural mesothelioma, proteomic analysis",

author = "Serena Lacerenza and Federica Ciregia and Laura Giusti and Alessandra Bonotti and Viviana Greco and Gino Giannaccini and Vanessa D{\textquoteright}Antongiovanni and Poupak Fallahi and Luisa Pieroni and Alfonso Cristaudo and Antonio Lucacchini and Mazzoni, \{Maria Rosa\} and Rudy Foddis",

year = "2020",

doi = "10.21873/cgp.20183",

language = "English",

pages = "225--236",

journal = "CANCER GENOMICS \& PROTEOMICS.",

issn = "1109-6535",

publisher = "Kapandriti, Attiki, Greece : International Institute of Anticancer Research, [2004]-",

}

Lacerenza, S, Ciregia, F, Giusti, L, Bonotti, A, Greco, V, Giannaccini, G, D’Antongiovanni, V, Fallahi, P, Pieroni, L, Cristaudo, A, Lucacchini, A, Mazzoni, MR & Foddis, R 2020, 'Putative Biomarkers for Malignant Pleural Mesothelioma Suggested by Proteomic Analysis of Cell Secretome.', CANCER GENOMICS & PROTEOMICS., pp. 225-236. https://doi.org/10.21873/cgp.20183

TY - JOUR

T1 - Putative Biomarkers for Malignant Pleural Mesothelioma Suggested by Proteomic Analysis of Cell Secretome.

AU - Lacerenza, Serena

AU - Ciregia, Federica

AU - Giusti, Laura

AU - Bonotti, Alessandra

AU - Greco, Viviana

AU - Giannaccini, Gino

AU - D’Antongiovanni, Vanessa

AU - Fallahi, Poupak

AU - Pieroni, Luisa

AU - Cristaudo, Alfonso

AU - Lucacchini, Antonio

AU - Mazzoni, Maria Rosa

AU - Foddis, Rudy

PY - 2020

Y1 - 2020

N2 - BACKGROUND:Malignant pleural mesothelioma (MPM) a rare neoplasm linked to asbestos exposure is characterized by a poor prognosis. Soluble mesothelin is currently considered the most specific diagnostic biomarker. The aim of the study was to identify novel biomarkers by proteomic analysis of two MPM cell lines secretome. MATERIALS AND METHODS:The protein patterns of MPM cells secretome were examined and compared to a non-malignant mesothelial cell line using two-dimensional gel electrophoresis coupled to mass spectrometry. Serum levels of candidate biomarkers were determined in MPM patients and control subjects. RESULTS:Two up-regulated proteins involved in cancer biology, prosaposin and quiescin Q6 sulfhydryl oxidase 1, were considered candidate biomarkers. Serum levels of both proteins were significantly higher in MPM patients than control subjects. Combining the data of each receiver-operating characteristic analysis predicted a good diagnostic accuracy. CONCLUSION:A panel of the putative biomarkers represents a promising tool for MPM diagnosis.

AB - BACKGROUND:Malignant pleural mesothelioma (MPM) a rare neoplasm linked to asbestos exposure is characterized by a poor prognosis. Soluble mesothelin is currently considered the most specific diagnostic biomarker. The aim of the study was to identify novel biomarkers by proteomic analysis of two MPM cell lines secretome. MATERIALS AND METHODS:The protein patterns of MPM cells secretome were examined and compared to a non-malignant mesothelial cell line using two-dimensional gel electrophoresis coupled to mass spectrometry. Serum levels of candidate biomarkers were determined in MPM patients and control subjects. RESULTS:Two up-regulated proteins involved in cancer biology, prosaposin and quiescin Q6 sulfhydryl oxidase 1, were considered candidate biomarkers. Serum levels of both proteins were significantly higher in MPM patients than control subjects. Combining the data of each receiver-operating characteristic analysis predicted a good diagnostic accuracy. CONCLUSION:A panel of the putative biomarkers represents a promising tool for MPM diagnosis.

KW - Malignant pleural mesothelioma

KW - proteomic analysis

KW - Malignant pleural mesothelioma

KW - proteomic analysis

UR - http://hdl.handle.net/10807/155270

U2 - 10.21873/cgp.20183

DO - 10.21873/cgp.20183

M3 - Article

SN - 1109-6535

SP - 225

EP - 236

JO - CANCER GENOMICS & PROTEOMICS.

JF - CANCER GENOMICS & PROTEOMICS.

ER -

Putative Biomarkers for Malignant Pleural Mesothelioma Suggested by Proteomic Analysis of Cell Secretome.

Abstract

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Keywords

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