Protease encoding microbial communities and protease activity of the rhizosphere and bulk soils of two maize lines with different N uptake efficiency

Edoardo Puglisi, Divyashri Baraniya, Maria Teresa Ceccherini, Giacomo Pietramellara, Laura Giagnoni, Mariarita Arenella, Paolo Nannipieri, Giancarlo Renella

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

This study was carried out to understand the interplay of plant Nitrogen Utilizing Efficiency (NUE) with protease activity and microbial proteolytic community composition in the rhizosphere and bulk soils. Protease activity, diversity and abundance of protease genes (using DGGE and qPCR respectively of two key bacterial protease encoding genes: alkaline metallo-peptidase (apr) and neutral-metallopeptidases (npr)) were monitored in both rhizosphere and bulk soils from two maize in-bred lines L05 and T250 with higher and lower NUE respectively, using a rhizobox approach. Illumina sequencing was employed to assess the diversity of proteolytic communities encoding for the above-mentioned protease genes. Our results show higher enzyme activity, higher abundance and diversity of proteolytic genes in L05 maize rhizosphere, with higher NUE than in T250 maize rhizosphere.
Original languageEnglish
Pages (from-to)176-179
Number of pages4
JournalSOIL BIOLOGY & BIOCHEMISTRY
Volume96
DOIs
Publication statusPublished - 2016

Keywords

  • Illumina sequencing
  • Microbiology
  • Nitrogen use efficiency
  • PCR-DGGE
  • Protease activity
  • Protease encoding genes
  • Rhizosphere
  • Soil Science

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