PHYSICOCHEMICAL AND FUNCTIONAL PROPERTIES OF PERINEREIS-CULTRIFERA (GRUBE) ERYTHROCRUORIN

E Chiancone; F Ascoli; Bruno Giardina; P Vecchini; E Antonini; M Musmeci; R Cina; M Zagra; V Damelio; G De Leo

doi:10.1016/0005-2795(77)90129-5

PHYSICOCHEMICAL AND FUNCTIONAL PROPERTIES OF PERINEREIS-CULTRIFERA (GRUBE) ERYTHROCRUORIN

E Chiancone, F Ascoli, Bruno Giardina, P Vecchini, E Antonini, M Musmeci, R Cina, M Zagra, V Damelio, G De Leo

Research output: Contribution to journal › Article

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Abstract

Perinereis erythrocruorin has the following physicochemical properties: , corresponding to a molecular weight around 2.7 · 106; minimum molecular weight (on the basis of the heme content) 23 700 ± 500; isoelectric point 5.1; α-helix content ≈ 40%. At alkaline pH values in the oxygenated form the 55-S molecules dissociate into subunits with a weight average sedimentation coefficient of 3 S, corresponding to a molecular weight ≈ 35 000. Deoxygenation of partially dissociated samples promotes association of the 3-S subunits into a 9 S component. The functional properties of Perinereis erythrocruorin are characterized by a low cooperativity in oxygen binding () at neutral pH. Cooperativity increases reversibly towards both the acid and alkaline pH range, irrespective of changes in molecular weight. This finding, taken together with the ultracentrifuge results, suggests that a subunit may represent the functional unit of the protein. The pH dependence of the oxygen affinity can be accounted for in terms of a single oxygen linked group with a of 8

Original language	English
Pages (from-to)	1-8
Number of pages	8
Journal	BIOCHIMICA ET BIOPHYSICA ACTA
Volume	494
DOIs	https://doi.org/10.1016/0005-2795(77)90129-5
Publication status	Published - 1977
Externally published	Yes

Keywords

ERYTHROCRUORIN

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10.1016/0005-2795(77)90129-5

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@article{c1ffa1e8a0fc4bbcb65777d6db1919d0,

title = "PHYSICOCHEMICAL AND FUNCTIONAL PROPERTIES OF PERINEREIS-CULTRIFERA (GRUBE) ERYTHROCRUORIN",

abstract = "Perinereis erythrocruorin has the following physicochemical properties: , corresponding to a molecular weight around 2.7 · 106; minimum molecular weight (on the basis of the heme content) 23 700 ± 500; isoelectric point 5.1; α-helix content ≈ 40%. At alkaline pH values in the oxygenated form the 55-S molecules dissociate into subunits with a weight average sedimentation coefficient of 3 S, corresponding to a molecular weight ≈ 35 000. Deoxygenation of partially dissociated samples promotes association of the 3-S subunits into a 9 S component. The functional properties of Perinereis erythrocruorin are characterized by a low cooperativity in oxygen binding () at neutral pH. Cooperativity increases reversibly towards both the acid and alkaline pH range, irrespective of changes in molecular weight. This finding, taken together with the ultracentrifuge results, suggests that a subunit may represent the functional unit of the protein. The pH dependence of the oxygen affinity can be accounted for in terms of a single oxygen linked group with a of 8",

keywords = "ERYTHROCRUORIN, ERYTHROCRUORIN",

author = "E Chiancone and F Ascoli and Bruno Giardina and P Vecchini and E Antonini and M Musmeci and R Cina and M Zagra and V Damelio and {De Leo}, G",

year = "1977",

doi = "10.1016/0005-2795(77)90129-5",

language = "English",

volume = "494",

pages = "1--8",

journal = "BIOCHIMICA ET BIOPHYSICA ACTA",

issn = "0006-3002",

publisher = "Elsevier BV:PO Box 211, 1000 AE Amsterdam Netherlands:011 31 20 4853757, 011 31 20 4853642, 011 31 20 4853641, EMAIL: [email protected], INTERNET: http://www.elsevier.nl, Fax: 011 31 20 4853598",

}

TY - JOUR

T1 - PHYSICOCHEMICAL AND FUNCTIONAL PROPERTIES OF PERINEREIS-CULTRIFERA (GRUBE) ERYTHROCRUORIN

AU - Chiancone, E

AU - Ascoli, F

AU - Giardina, Bruno

AU - Vecchini, P

AU - Antonini, E

AU - Musmeci, M

AU - Cina, R

AU - Zagra, M

AU - Damelio, V

AU - De Leo, G

PY - 1977

Y1 - 1977

N2 - Perinereis erythrocruorin has the following physicochemical properties: , corresponding to a molecular weight around 2.7 · 106; minimum molecular weight (on the basis of the heme content) 23 700 ± 500; isoelectric point 5.1; α-helix content ≈ 40%. At alkaline pH values in the oxygenated form the 55-S molecules dissociate into subunits with a weight average sedimentation coefficient of 3 S, corresponding to a molecular weight ≈ 35 000. Deoxygenation of partially dissociated samples promotes association of the 3-S subunits into a 9 S component. The functional properties of Perinereis erythrocruorin are characterized by a low cooperativity in oxygen binding () at neutral pH. Cooperativity increases reversibly towards both the acid and alkaline pH range, irrespective of changes in molecular weight. This finding, taken together with the ultracentrifuge results, suggests that a subunit may represent the functional unit of the protein. The pH dependence of the oxygen affinity can be accounted for in terms of a single oxygen linked group with a of 8

AB - Perinereis erythrocruorin has the following physicochemical properties: , corresponding to a molecular weight around 2.7 · 106; minimum molecular weight (on the basis of the heme content) 23 700 ± 500; isoelectric point 5.1; α-helix content ≈ 40%. At alkaline pH values in the oxygenated form the 55-S molecules dissociate into subunits with a weight average sedimentation coefficient of 3 S, corresponding to a molecular weight ≈ 35 000. Deoxygenation of partially dissociated samples promotes association of the 3-S subunits into a 9 S component. The functional properties of Perinereis erythrocruorin are characterized by a low cooperativity in oxygen binding () at neutral pH. Cooperativity increases reversibly towards both the acid and alkaline pH range, irrespective of changes in molecular weight. This finding, taken together with the ultracentrifuge results, suggests that a subunit may represent the functional unit of the protein. The pH dependence of the oxygen affinity can be accounted for in terms of a single oxygen linked group with a of 8

KW - ERYTHROCRUORIN

UR - http://hdl.handle.net/10807/6831

U2 - 10.1016/0005-2795(77)90129-5

DO - 10.1016/0005-2795(77)90129-5

M3 - Article

SN - 0006-3002

VL - 494

SP - 1

EP - 8

JO - BIOCHIMICA ET BIOPHYSICA ACTA

JF - BIOCHIMICA ET BIOPHYSICA ACTA

ER -

PHYSICOCHEMICAL AND FUNCTIONAL PROPERTIES OF PERINEREIS-CULTRIFERA (GRUBE) ERYTHROCRUORIN

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Keywords

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