Perinatal derivatives: How to best validate their immunomodulatory functions

Andrea Papait, Antonietta Rosa Silini, Maria Gazouli, Ricardo Malvicini, Maurizio Muraca, Lorraine O’Driscoll, Natalia Pacienza, Wei Seong Toh, Gustavo Yannarelli, Peter Ponsaerts, Ornella Parolini, Günther Eissner, Michela Pozzobon, Sai Kiang Lim, Bernd Giebel*

*Corresponding author

Research output: Contribution to journalArticle

Abstract

Perinatal tissues, mainly the placenta and umbilical cord, contain a variety of different somatic stem and progenitor cell types, including those of the hematopoietic system, multipotent mesenchymal stromal cells (MSCs), epithelial cells and amnion epithelial cells. Several of these perinatal derivatives (PnDs), as well as their secreted products, have been reported to exert immunomodulatory therapeutic and regenerative functions in a variety of pre-clinical disease models. Following experience with MSCs and their extracellular vesicle (EV) products, successful clinical translation of PnDs will require robust functional assays that are predictive for the relevant therapeutic potency. Using the examples of T cell and monocyte/macrophage assays, we here discuss several assay relevant parameters for assessing the immunomodulatory activities of PnDs. Furthermore, we highlight the need to correlate the in vitro assay results with preclinical or clinical outcomes in order to ensure valid predictions about the in vivo potency of therapeutic PnD cells/products in individual disease settings.
Original languageEnglish
Pages (from-to)N/A-N/A
JournalFrontiers in Bioengineering and Biotechnology
Volume10
DOIs
Publication statusPublished - 2022

Keywords

  • exosomes
  • extracellular vesicles
  • functional assays
  • immunomodulation
  • mechanisms of action
  • mesenchymal stromal cells
  • microvesicles
  • perinatal derivatives

Fingerprint

Dive into the research topics of 'Perinatal derivatives: How to best validate their immunomodulatory functions'. Together they form a unique fingerprint.

Cite this