Overexpression of PRV-1 gene in polycythemia rubra vera and essential thrombocythemia

Maurizio Martini, Luciana Teofili, Luigi Maria Larocca

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (Scopus)

Abstract

The polycythemia rubra vera 1 gene (PRV-1), a member of the urokinase-type plasminogen activator receptor superfamily, is overexpressed in granulocytes isolated from the peripheral blood of patients with polycythemia vera (PV) and essential thrombocythemia (ET). PRV-1 overexpression is the first reliable molecular marker of these myeloproliferative disorders, and its detection allows us to discriminate PV and ET from secondary erythrocytosis and thrombocytosis. PRV-1 overexpression can be investigated by several techniques, including Northern analysis, reverse-transcription (RT)-polymerase chain reaction (PCR), and real-time PCR. Among these, RT-PCR is the most rapid, reliable, and feasible method for the detection of PRV-1 overexpression in highly purified peripheral blood granulocytes.
Original languageEnglish
Title of host publicationMethods in Molecular Medicine
Pages265-273
Number of pages9
Publication statusPublished - 2006

Keywords

  • Diagnosis, Differential
  • GPI-Linked Proteins
  • Gene Expression Regulation
  • Granulocytes
  • Humans
  • Isoantigens
  • Membrane Glycoproteins
  • Polycythemia Vera
  • RNA
  • RNA, Messenger
  • Receptors, Cell Surface
  • Reverse Transcriptase Polymerase Chain Reaction
  • Thrombocythemia, Essential

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