TY - JOUR
T1 - Optimized ultrasound-assisted extraction of phenolic compounds from Thymus comosus Heuff. ex Griseb. et Schenk (wild thyme) and their bioactive potential
AU - Babotă, Mihai
AU - Frumuzachi, Oleg
AU - Gâvan, Alexandru
AU - Iacoviță, Cristian
AU - Pinela, José
AU - Barros, Lillian
AU - Ferreira, Isabel C.F.R.
AU - Zhang, Leilei
AU - Lucini, Luigi
AU - Rocchetti, Gabriele
AU - Tanase, Corneliu
AU - Crișan, Gianina
AU - Mocan, Andrei
PY - 2022
Y1 - 2022
N2 - An optimized ultrasound-assisted extractive method was developed to obtain a polyphenol-enriched extract from the aerial parts of Thymus comosus Heuff. ex Griseb. et Schenk. Optimization process was conducted based on Design of Experiment (DoE) principles, determining the influence of three independent variables (time, ultrasound amplitude, ethanol concentration) on the total phenolic content of the extract (dependent variable). Additionally, the phenolic composition of the extract was characterized through UHPLC-HRMS, revealing beside the most abundant flavonoid-type compounds the presence of salvianolic acids C, D and L in high amounts. Phytochemical profile of the extract was correlated with its antioxidant activity (tested through five complementary assays) and enzyme-inhibitory potential, showing important antiglucosidase and anticholinesterase effects. Overall, it was concluded that the developed method is suitable for obtaining a good recovery of both phenolic and non-phenolic compounds from Thymus comosus aerial parts, and their presence in the optimized extract is responsible for its pharmacological potential.
AB - An optimized ultrasound-assisted extractive method was developed to obtain a polyphenol-enriched extract from the aerial parts of Thymus comosus Heuff. ex Griseb. et Schenk. Optimization process was conducted based on Design of Experiment (DoE) principles, determining the influence of three independent variables (time, ultrasound amplitude, ethanol concentration) on the total phenolic content of the extract (dependent variable). Additionally, the phenolic composition of the extract was characterized through UHPLC-HRMS, revealing beside the most abundant flavonoid-type compounds the presence of salvianolic acids C, D and L in high amounts. Phytochemical profile of the extract was correlated with its antioxidant activity (tested through five complementary assays) and enzyme-inhibitory potential, showing important antiglucosidase and anticholinesterase effects. Overall, it was concluded that the developed method is suitable for obtaining a good recovery of both phenolic and non-phenolic compounds from Thymus comosus aerial parts, and their presence in the optimized extract is responsible for its pharmacological potential.
KW - Design of Experiments
KW - Optimisation
KW - Phenolic compounds
KW - Thymus comosus Heuff. ex Griseb. et Schenk
KW - Ultrasound-assisted extraction
KW - Design of Experiments
KW - Optimisation
KW - Phenolic compounds
KW - Thymus comosus Heuff. ex Griseb. et Schenk
KW - Ultrasound-assisted extraction
UR - http://hdl.handle.net/10807/232248
U2 - 10.1016/j.ultsonch.2022.105954
DO - 10.1016/j.ultsonch.2022.105954
M3 - Article
SN - 1350-4177
VL - 84
SP - N/A-N/A
JO - Ultrasonics Sonochemistry
JF - Ultrasonics Sonochemistry
ER -