NF-KB localization in multiple myeloma plasma cells and mesenchymal cells

Ruggero De Maria Marchiano, Concetta Conticello, Raffaella Giuffrida, Luana Adamo, Gabriele Anastasi, Daniela Martinetti, Edvige Salomone, Cristina Colarossi, Gabriella Amato, Ausilia Gorgone, Alessandra Romano, Gioacchin Iannolo, Rosario Giustolisi, Massimo Gulisano, Francesco Di Raimondo

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Several reports demonstrated that the activation of Nuclear Factor-kappa B NF-κB is essential for the pathogenesis of multiple myeloma (MM). We analyzed the nuclear localization of NF-κB in MM-cells derived from 60 different patients with MM at presentation and in relapse, as well as in three myeloma cell lines. Nuclear localization (the active form) of NF-κB was detected in only one MM-sample from a refractory patient and in two samples from relapsed patients, while all the other samples, including the MM-cell lines, almost exclusively express the cytoplasmic (inactive) form of NF-κB. In mesenchymal cells from MM-patients NF-κB was clearly present in the nucleus. In addition, the proteasome inhibitor Bortezomib, which is described to antagonize NF-κB activity, had a consistent antitumor activity against both chemoresistant and chemosensitive MM-cells, regardless the NF-κB localization, thus suggesting the existence of other molecular targets of proteasome inhibitors in MM. © 2010 Elsevier Ltd.
Original languageEnglish
Pages (from-to)52-60
Number of pages9
JournalLeukemia Research
Volume35
DOIs
Publication statusPublished - 2011

Keywords

  • Antineoplastic Agents
  • Blotting, Western
  • Boronic Acids
  • Bortezomib
  • Cancer Research
  • Cell Line, Tumor
  • Flow Cytometry
  • Hematology
  • Humans
  • Immunohistochemistry
  • Mesenchymal cells
  • Mesoderm
  • Multiple Myeloma
  • Multiple myeloma
  • NF-kappa B
  • NF-κB
  • Oncology
  • Plasma Cells
  • Pyrazines

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