Neuroglobin expression and oxidant/antioxidant balance after graded traumatic brain injury in the rat.

Valentina Di Pietro, Giacomo Lazzarino, Angela Maria Amorini, Barbara Tavazzi, Serafina D'Urso, Salvatore Antonio Longo, Roberto Vagnozzi, Stefano Signoretti, Maria Elisabetta Clementi, Bruno Giardina, Giuseppe Lazzarino, Antonio Belli

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)

Abstract

Neuroglobin is a neuron-specific hexacoordinated globin capable of binding various ligands, including O2, NO, and CO, the biological function of which is still uncertain. Various studies seem to indicate that neuroglobin is a neuroprotective agent when overexpressed, acting as a potent inhibitor of oxidative and nitrosative stress. In this study, we evaluated the pathophysiological response of the neuroglobin gene and protein expression in the cerebral tissue of rats sustaining traumatic brain injury of differing severity, while simultaneously measuring the oxidant/antioxidant balance. Two levels of trauma (mild and severe) were induced in anesthetized animals using the weight-drop model of diffuse axonal injury. Rats were then sacrificed at 6, 12, 24, 48, and 120h after traumatic brain injury, and the gene and protein expression of neuroglobin and the concentrations of malondialdehyde (as a parameter representative of reactive oxygen species-mediated damage), nitrite + nitrate (indicative of NO metabolism), ascorbate, and glutathione (GSH) were determined in the brain tissue. Results indicated that mild traumatic brain injury, although causing a reversible increase in oxidative/nitrosative stress (increase in malondialdehyde and nitrite + nitrate) and an imbalance in antioxidants (decrease in ascorbate and GSH), did not induce any change in neuroglobin. Conversely, severe traumatic brain injury caused an over nine- and a fivefold increase in neuroglobin gene and protein expression, respectively, as well as a remarkable increase in oxidative/nitrosative stress and depletion of antioxidants. The results of this study, showing a lack of effect in mild traumatic brain injury as well as asynchronous time course changes in neuroglobin expression, oxidative/nitrosative stress, and antioxidants in severe traumatic brain injury, do not seem to support the role of neuroglobin as an endogenous neuroprotective antioxidant agent, at least under pathophysiological conditions.
Original languageEnglish
Pages (from-to)258-264
Number of pages7
JournalFree Radic Biol Med.
Volume2014
DOIs
Publication statusPublished - 2014

Keywords

  • Neuroglobin
  • oxidant/antioxidant balance
  • traumatic brain injury

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