Multiple malaria infection in a pregnant woman from Nigeria: detection by multiplex PCR

Luca Masucci, Francesca Bugli, Fernando Damiano, Giovanni Fadda, Rosalia Graffeo, Giovanni Federico, Carmen Pinnetti

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

For the last 100 years, diagnosis of malaria has been based on examination of Giemsa-stained thick and thin blood films under the microscope. This is a time-consuming procedure which often fails to correctly diagnose the infecting species especially when carried out by inexperienced technicians or when blood levels of parasite are low. Rapid Diagnostic Tests (RDTs) for antigen detection can distinguish between Plasmodium falciparum and Plasmodium vivax but cannot identify the species present in mixed infections. In the case reported here we used multiplex PCR to investigate suspected mixed infection in a pregnant woman from Nigeria. The results suggest that the method used is highly specific and can be very sensitive and that it has several advantages with respect to microscopy and RTDs
Original languageEnglish
Pages (from-to)565-567
Number of pages3
JournalNew Microbiologica
Volume31
Publication statusPublished - 2008

Keywords

  • Malaria
  • PCR
  • multiple infection

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