Monosodium Urate Crystals Promote Innate Anti-Mycobacterial Immunity and Improve BCG Efficacy as a Vaccine against Tuberculosis

Ivana Palucci, Giovanni Delogu, Francesco Taus, Marilina B. Santucci, Emanuela Greco, Sabrina Mariotti, Noemi Poerio, Roberto Nisini, Maurizio Fraziano

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

A safer and more effective anti-Tuberculosis vaccine is still an urgent need. We probed the effects of monosodium urate crystals (MSU) on innate immunity to improve the Bacille Calmette-Guerin (BCG) vaccination. Results showed that in vitro MSU cause an enduring macrophage stimulation of the anti-mycobacterial response, measured as intracellular killing, ROS production and phagolysosome maturation. The contribution of MSU to anti-mycobacterial activity was also shown in vivo. Mice vaccinated in the presence of MSU showed a lower number of BCG in lymph nodes draining the vaccine inoculation site, in comparison to mice vaccinated without MSU. Lastly, we showed that MSU improved the efficacy of BCG vaccination in mice infected with Mycobacterium tuberculosis (MTB), measured in terms of lung and spleen MTB burden. These results demonstrate that the use of MSU as adjuvant may represent a novel strategy to enhance the efficacy of BCG vaccination.
Original languageEnglish
Pages (from-to)e0127279-e0127279
Number of pages16
JournalPLoS One
Volume10
DOIs
Publication statusPublished - 2015

Keywords

  • tuberculosis
  • vaccine

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