Mononuclear cell collection for extracorporeal photopheresis: Concentrate characteristics for off-line UV-A irradiation procedure

Nicola Piccirillo, Patrizia Chiusolo, Simona Sica, Gina Zini, Rossana Putzulu, Giuseppina Massini

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background and objective: Extracorporeal photopheresis (ECP) is the most represented cell therapy for treatment of cutaneous T-cell lymphoma, graft-versus host disease and organ rejection. We analyzed our experience in ECP using 2 cell separators (Cobe Spectra and Spectra Optia) focusing on leukapheretic product characteristics, UV-A irradiation procedure and entire ECP process. Materials and methods: We collected data of patients undergoing ECP between January 2012 and February 2015 in order to evaluate collection procedures performed using Cobe Spectra and Spectra Optia, mononuclear cell product, UV-A photoirradiation procedure by Pit System. Results: We performed 484 ECP procedures in 27 patients. Cobe-derived mononuclear cell products were characterized by higher cell yields while Optia-derived mononuclear cell products were characterized by smaller volume, comparable mononuclear cell content but lower erythrocytes, granulocytes, and platelets contamination. Conclusion: Our study confirms good results for both cell separators. Blood volume processed being equal, Cobe collects a number of total nucleated cells significantly higher than Optia. Optia, collecting only target cells without significant erythrocytes, granulocytes and platelets contamination, is able to collect a leukapheretic product particularly suitable for ECP.
Original languageEnglish
Pages (from-to)N/A-N/A
JournalJournal of Clinical Apheresis
Volume2017
DOIs
Publication statusPublished - 2017

Keywords

  • Concentrate characteristics
  • Erythrocytes contamination
  • Extracorporeal photopheresis (ECP)
  • Hematology
  • Mononuclear cells collection

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