Monitoring nutrient signaling through the longevity protein p66(SHC¹)

Sofia Chiatamone Ranieri; Giovambattista Pani

doi:10.1007/978-1-62703-239-1_23

Monitoring nutrient signaling through the longevity protein p66(SHC¹)

Sofia Chiatamone Ranieri, Giovambattista Pani

Istituto di Ricovero e Cura a Carattere Scientifico

Research output: Contribution to journal › Article › peer-review

Abstract

Nutrient availability and nutrient-dependent biochemical signals represent major determinants of cellular senescence and organismal aging. The present chapter describes simple procedures to reliably evaluate the response of cultured cell to nutrients through the longevity protein p66(SHC1) and the mTOR/S6K cascade, which might be used to study cellular senescence and its chemical modulation by pharmaceutical agents in vitro.

Original language	English
Pages (from-to)	341-353
Number of pages	13
Journal	METHODS IN MOLECULAR BIOLOGY
Volume	965
DOIs	https://doi.org/10.1007/978-1-62703-239-1_23
Publication status	Published - 2013

Keywords

Aging
Electrophoresis
Gene Knockdown Techniques
HEK293 Cells
Humans
Longevity
Phosphorylation
Shc Signaling Adaptor Proteins
Signal Transduction
Staining and Labeling
Transfection

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10.1007/978-1-62703-239-1_23

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abstract = "Nutrient availability and nutrient-dependent biochemical signals represent major determinants of cellular senescence and organismal aging. The present chapter describes simple procedures to reliably evaluate the response of cultured cell to nutrients through the longevity protein p66(SHC1) and the mTOR/S6K cascade, which might be used to study cellular senescence and its chemical modulation by pharmaceutical agents in vitro.",

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T1 - Monitoring nutrient signaling through the longevity protein p66(SHC¹)

AU - Ranieri, Sofia Chiatamone

AU - Pani, Giovambattista

PY - 2013

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N2 - Nutrient availability and nutrient-dependent biochemical signals represent major determinants of cellular senescence and organismal aging. The present chapter describes simple procedures to reliably evaluate the response of cultured cell to nutrients through the longevity protein p66(SHC1) and the mTOR/S6K cascade, which might be used to study cellular senescence and its chemical modulation by pharmaceutical agents in vitro.

AB - Nutrient availability and nutrient-dependent biochemical signals represent major determinants of cellular senescence and organismal aging. The present chapter describes simple procedures to reliably evaluate the response of cultured cell to nutrients through the longevity protein p66(SHC1) and the mTOR/S6K cascade, which might be used to study cellular senescence and its chemical modulation by pharmaceutical agents in vitro.

KW - Aging

KW - Electrophoresis

KW - Gene Knockdown Techniques

KW - HEK293 Cells

KW - Humans

KW - Longevity

KW - Phosphorylation

KW - Shc Signaling Adaptor Proteins

KW - Signal Transduction

KW - Staining and Labeling

KW - Transfection

KW - Aging

KW - Electrophoresis

KW - Gene Knockdown Techniques

KW - HEK293 Cells

KW - Humans

KW - Longevity

KW - Phosphorylation

KW - Shc Signaling Adaptor Proteins

KW - Signal Transduction

KW - Staining and Labeling

KW - Transfection

UR - http://hdl.handle.net/10807/52408

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DO - 10.1007/978-1-62703-239-1_23

M3 - Article

SN - 0097-0816

VL - 965

SP - 341

EP - 353

JO - METHODS IN MOLECULAR BIOLOGY

JF - METHODS IN MOLECULAR BIOLOGY

ER -

Monitoring nutrient signaling through the longevity protein p66(SHC¹)

Abstract

Keywords

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