Abstract
The use of the (GTG)5 oligonucleotide, a repetitive marker in the Mycobacterium tuberculosis chromosome, as a primer in association with an IS6110 outlooking primer has been successfully applied to a PCR-based fingerprinting method. This method classified 62 strains of M. tuberculosis, isolated from human immunodeficiency virus-seropositive and -seronegative patients in different regions of Italy and Pakistan, as having 53 different patterns. The results were compared with traditional IS6110 fingerprinting, by which 47 distinct patterns were observed.
| Original language | English |
|---|---|
| Pages (from-to) | 1825-1828 |
| Number of pages | 4 |
| Journal | Journal of Clinical Microbiology |
| Volume | 34 |
| Publication status | Published - 1996 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- AIDS-Related Opportunistic Infections
- Bacterial Typing Techniques
- Base Sequence
- DNA Fingerprinting
- DNA Primers
- DNA Transposable Elements
- Evaluation Studies as Topic
- Genetic Markers
- HIV Seronegativity
- HIV Seropositivity
- Humans
- Italy
- Molecular Epidemiology
- Mycobacterium tuberculosis
- Pakistan
- Polymerase Chain Reaction
- Repetitive Sequences, Nucleic Acid
- Tuberculosis, Pulmonary
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