TY - JOUR
T1 - Molecular epidemiology of Mycobacterium tuberculosis strains isolated from different regions of Italy and Pakistan
AU - Sechi, La
AU - Zanetti, S
AU - Delogu, Giovanni
AU - Montinaro, B
AU - Sanna, A
AU - Fadda, Giovanni
PY - 1996
Y1 - 1996
N2 - The use of the (GTG)5 oligonucleotide, a repetitive marker in the Mycobacterium tuberculosis chromosome, as a primer in association with an IS6110 outlooking primer has been successfully applied to a PCR-based fingerprinting method. This method classified 62 strains of M. tuberculosis, isolated from human immunodeficiency virus-seropositive and -seronegative patients in different regions of Italy and Pakistan, as having 53 different patterns. The results were compared with traditional IS6110 fingerprinting, by which 47 distinct patterns were observed.
AB - The use of the (GTG)5 oligonucleotide, a repetitive marker in the Mycobacterium tuberculosis chromosome, as a primer in association with an IS6110 outlooking primer has been successfully applied to a PCR-based fingerprinting method. This method classified 62 strains of M. tuberculosis, isolated from human immunodeficiency virus-seropositive and -seronegative patients in different regions of Italy and Pakistan, as having 53 different patterns. The results were compared with traditional IS6110 fingerprinting, by which 47 distinct patterns were observed.
KW - AIDS-Related Opportunistic Infections
KW - Bacterial Typing Techniques
KW - Base Sequence
KW - DNA Fingerprinting
KW - DNA Primers
KW - DNA Transposable Elements
KW - Evaluation Studies as Topic
KW - Genetic Markers
KW - HIV Seronegativity
KW - HIV Seropositivity
KW - Humans
KW - Italy
KW - Molecular Epidemiology
KW - Mycobacterium tuberculosis
KW - Pakistan
KW - Polymerase Chain Reaction
KW - Repetitive Sequences, Nucleic Acid
KW - Tuberculosis, Pulmonary
KW - AIDS-Related Opportunistic Infections
KW - Bacterial Typing Techniques
KW - Base Sequence
KW - DNA Fingerprinting
KW - DNA Primers
KW - DNA Transposable Elements
KW - Evaluation Studies as Topic
KW - Genetic Markers
KW - HIV Seronegativity
KW - HIV Seropositivity
KW - Humans
KW - Italy
KW - Molecular Epidemiology
KW - Mycobacterium tuberculosis
KW - Pakistan
KW - Polymerase Chain Reaction
KW - Repetitive Sequences, Nucleic Acid
KW - Tuberculosis, Pulmonary
UR - http://hdl.handle.net/10807/3131
M3 - Article
SN - 0095-1137
VL - 34
SP - 1825
EP - 1828
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
ER -
