Methodological considerations for large-scale breath analysis studies: lessons from the U-BIOPRED severe asthma project

Waqar M. Ahmed; Paul Brinkman; Hans Weda; Hugo H. Knobel; Yun Xu; Tamara M. Nijsen; Royston Goodacre; Nicholas Rattray; Teunis J. Vink; Marco Santonico; Giorgio Pennazza; Paolo Montuschi; Peter J. Sterk; Stephen J. Fowler

doi:10.1088/1752-7163/aae557

Methodological considerations for large-scale breath analysis studies: lessons from the U-BIOPRED severe asthma project

Waqar M. Ahmed, Paul Brinkman, Hans Weda, Hugo H. Knobel, Yun Xu, Tamara M. Nijsen, Royston Goodacre, Nicholas Rattray, Teunis J. Vink, Marco Santonico, Giorgio Pennazza, Paolo Montuschi, Peter J. Sterk, Stephen J. Fowler

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Methods for breath sampling and analysis require robust quality assessment to minimise the risk of false discoveries. Planning large-scale multi-site breath metabolite profiling studies also requires careful consideration of systematic and random variation as a result of sampling and analysis techniques. In this study we use breath sample data from the recent U-BIOPRED cohort to evaluate and discuss some important methodological considerations such as batch variation and correction, variation between sites, storage and transportation, as well as inter-instrument analytical differences. Based on this we provide a summary of recommended best practices for new large scale multi-site studies

Original language	English
Pages (from-to)	N/A-N/A
Journal	Journal of Breath Research
Volume	13
DOIs	https://doi.org/10.1088/1752-7163/aae557
Publication status	Published - 2019

Keywords

breath analysis
severe asthma

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10.1088/1752-7163/aae557

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Ahmed, W. M., Brinkman, P., Weda, H., Knobel, H. H., Xu, Y., Nijsen, T. M., Goodacre, R., Rattray, N., Vink, T. J., Santonico, M., Pennazza, G., Montuschi, P., Sterk, P. J., & Fowler, S. J. (2019). Methodological considerations for large-scale breath analysis studies: lessons from the U-BIOPRED severe asthma project. Journal of Breath Research, 13, N/A-N/A. https://doi.org/10.1088/1752-7163/aae557

@article{60ed7b02b6454b39b7481c4dac37efd9,

title = "Methodological considerations for large-scale breath analysis studies: lessons from the U-BIOPRED severe asthma project",

abstract = "Methods for breath sampling and analysis require robust quality assessment to minimise the risk of false discoveries. Planning large-scale multi-site breath metabolite profiling studies also requires careful consideration of systematic and random variation as a result of sampling and analysis techniques. In this study we use breath sample data from the recent U-BIOPRED cohort to evaluate and discuss some important methodological considerations such as batch variation and correction, variation between sites, storage and transportation, as well as inter-instrument analytical differences. Based on this we provide a summary of recommended best practices for new large scale multi-site studies",

keywords = "breath analysis, severe asthma, breath analysis, severe asthma",

author = "Ahmed, {Waqar M.} and Paul Brinkman and Hans Weda and Knobel, {Hugo H.} and Yun Xu and Nijsen, {Tamara M.} and Royston Goodacre and Nicholas Rattray and Vink, {Teunis J.} and Marco Santonico and Giorgio Pennazza and Paolo Montuschi and Sterk, {Peter J.} and Fowler, {Stephen J.}",

year = "2019",

doi = "10.1088/1752-7163/aae557",

language = "English",

volume = "13",

pages = "N/A--N/A",

journal = "Journal of Breath Research",

issn = "1752-7155",

publisher = "Bristol : IOP, 2007-.",

}

Ahmed, WM, Brinkman, P, Weda, H, Knobel, HH, Xu, Y, Nijsen, TM, Goodacre, R, Rattray, N, Vink, TJ, Santonico, M, Pennazza, G, Montuschi, P, Sterk, PJ & Fowler, SJ 2019, 'Methodological considerations for large-scale breath analysis studies: lessons from the U-BIOPRED severe asthma project', Journal of Breath Research, vol. 13, pp. N/A-N/A. https://doi.org/10.1088/1752-7163/aae557

TY - JOUR

T1 - Methodological considerations for large-scale breath analysis studies: lessons from the U-BIOPRED severe asthma project

AU - Ahmed, Waqar M.

AU - Brinkman, Paul

AU - Weda, Hans

AU - Knobel, Hugo H.

AU - Xu, Yun

AU - Nijsen, Tamara M.

AU - Goodacre, Royston

AU - Rattray, Nicholas

AU - Vink, Teunis J.

AU - Santonico, Marco

AU - Pennazza, Giorgio

AU - Montuschi, Paolo

AU - Sterk, Peter J.

AU - Fowler, Stephen J.

PY - 2019

Y1 - 2019

N2 - Methods for breath sampling and analysis require robust quality assessment to minimise the risk of false discoveries. Planning large-scale multi-site breath metabolite profiling studies also requires careful consideration of systematic and random variation as a result of sampling and analysis techniques. In this study we use breath sample data from the recent U-BIOPRED cohort to evaluate and discuss some important methodological considerations such as batch variation and correction, variation between sites, storage and transportation, as well as inter-instrument analytical differences. Based on this we provide a summary of recommended best practices for new large scale multi-site studies

AB - Methods for breath sampling and analysis require robust quality assessment to minimise the risk of false discoveries. Planning large-scale multi-site breath metabolite profiling studies also requires careful consideration of systematic and random variation as a result of sampling and analysis techniques. In this study we use breath sample data from the recent U-BIOPRED cohort to evaluate and discuss some important methodological considerations such as batch variation and correction, variation between sites, storage and transportation, as well as inter-instrument analytical differences. Based on this we provide a summary of recommended best practices for new large scale multi-site studies

KW - breath analysis

KW - severe asthma

KW - breath analysis

KW - severe asthma

UR - http://hdl.handle.net/10807/134877

U2 - 10.1088/1752-7163/aae557

DO - 10.1088/1752-7163/aae557

M3 - Article

SN - 1752-7155

VL - 13

SP - N/A-N/A

JO - Journal of Breath Research

JF - Journal of Breath Research

ER -

Methodological considerations for large-scale breath analysis studies: lessons from the U-BIOPRED severe asthma project

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Keywords

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