Mesenchymal differentiation of glioblastoma stem cells

L Ricci Vitiani, Roberto Pallini, Luigi Maria Larocca, Dg Lombardi, M Signore, Francesco Pierconti, Giovanna Petrucci, Nicola Montano, Giulio Maira, R. De Maria

Research output: Contribution to journalArticle

82 Citations (Scopus)


Glioblastoma multiforme is a severe form of cancer most likely arising from the transformation of stem or progenitor cells resident in the brain. Although the tumorigenic population in glioblastoma is defined as composed by cancer stem cells (CSCs), the cellular target of the transformation hit remains to be identified. Glioma stem cells (SCs) are thought to have a differentiation potential restricted to the neural lineage. However, using orthotopic versus heterotopic xenograft models and in vitro differentiation assays, we found that a subset of glioblastomas contained CSCs with both neural and mesenchymal potential. Subcutaneous injection of CSCs or single CSC clones from two of seven patients produced tumor xenografts containing osteo-chondrogenic areas in the context of glioblastoma-like tumor lesions. Moreover, CSC clones from four of seven cases generated both neural and chondrogenic cells in vitro. Interestingly, mesenchymal differentiation of the tumor xenografts was associated with reduction of both growth rate and mitotic index. These findings suggest that in a subclass of glioblastomas the tumorigenic hit occurs on a multipotent stem cell, which may reveal its plasticity under specific environmental stimuli. The discovery of such biological properties might provide considerable information to the development of new therapeutic strategies aimed at forcing glioblastoma stem cell differentiation.
Original languageEnglish
Pages (from-to)1491-1498
Number of pages1498
JournalCell Death and Differentiation
Publication statusPublished - 2008


  • Adult
  • Animals
  • Brain Neoplasms
  • Cell Differentiation
  • Clone Cells
  • Female
  • Glioblastoma
  • Humans
  • Male
  • Mesoderm
  • Mice
  • Mice, SCID
  • Middle Aged
  • Neoplastic Stem Cells
  • Neurons
  • Xenograft Model Antitumor Assays


Dive into the research topics of 'Mesenchymal differentiation of glioblastoma stem cells'. Together they form a unique fingerprint.

Cite this