Mechanisms of desflurane-induced preconditioning in isolated human right atria in vitro

Massimo Massetti, Jean Luc Hanouz, Alexandra Yvon, Olivier Lepage, Gérard Babatasi, André Khayat, Henri Bricard, Jean Louis Gérard

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

BACKGROUND: The authors examined the role of adenosine triphosphate-sensitive potassium (K(ATP)) channels, adenosine A1 receptor, and alpha and beta adrenoceptors in desflurane-induced preconditioning in human myocardium, in vitro. METHODS: The authors recorded isometric contraction of human right atrial trabeculae suspended in oxygenated Tyrode's solution (34 degrees C; stimulation frequency, 1 Hz). Before a 30-min anoxic period, 3, 6, and 9% desflurane was administered during 15 min. Desflurane, 6%, was also administered in the presence of 10 microm glibenclamide, a K(ATP) channels antagonist; 10 microm HMR 1098, a sarcolemmal K(ATP) channel antagonist; 800 microm 5-hydroxy-decanoate (5-HD), a mitochondrial K(ATP) channel antagonist; 1 microm phentolamine, an alpha-adrenoceptor antagonist; 1 microm propranolol, a beta-adrenoceptor antagonist; and 100 nm 8-cyclopentyl-1,3-dipropylxanthine (DPX), the adenosine A1 receptor antagonist. Developed force at the end of a 60-min reoxygenation period was compared (mean +/- SD). RESULTS: Desflurane at 3% (95 +/- 13% of baseline), 6% (86 +/- 6% of baseline), and 9% (82 +/- 6% of baseline) enhanced the recovery of force after 60 min of reoxygenation as compared with the control group (50 +/- 11% of baseline). Glibenclamide (60 +/- 12% of baseline), 5-HD (57 +/- 21% of baseline), DPX (63 +/- 19% of baseline), phentolamine (56 +/- 20% of baseline), and propranolol (63 +/- 13% of baseline) abolished desflurane-induced preconditioning. In contrast, HMR 1098 (85 +/- 12% of baseline) did not modify desflurane-induced preconditioning. CONCLUSIONS: In vitro, desflurane preconditions human myocardium against simulated ischemia through activation of mitochondrial K(ATP) channels, adenosine A1 receptor, and alpha and beta adrenoceptors.
Original languageEnglish
Pages (from-to)33-41
Number of pages9
JournalAnesthesiology
Volume97
Publication statusPublished - 2002

Keywords

  • Adenosine Triphosphate
  • Anesthetics, Inhalation
  • Glyburide
  • Heart
  • Humans
  • In Vitro Techniques
  • Ischemic Preconditioning, Myocardial
  • Isoflurane
  • Myocardial Contraction
  • Potassium Channels
  • Receptors, Adrenergic, alpha
  • Receptors, Adrenergic, beta
  • Receptors, Purinergic P1

Fingerprint Dive into the research topics of 'Mechanisms of desflurane-induced preconditioning in isolated human right atria in vitro'. Together they form a unique fingerprint.

Cite this