Mechanisms of antibiotic resistance to enrofloxacin in uropathogenic Escherichia coli in dog

Viviana Greco, Andrea Urbani, Paola Roncada, Cristian Piras, Alessio Soggiu, Piera Anna Martino, Federica Del Chierico, Lorenza Putignani, Jarlath E. Nally, Luigi Bonizzi

Research output: Contribution to journalArticle

27 Citations (Scopus)


Escherichia coli (E. coli) urinary tract infections (UTIs) are becoming a serious problem both for pets and humans (zoonosis) due to the close contact and to the increasing resistance to antibiotics. This study has been performed in order to unravel the mechanism of induced enrofloxacin resistance in canine E. coli isolates that represent a good tool to study this pathology. The isolated E. coli has been induced with enrofloxacin and studied through 2D DIGE and shotgun MS. Discovered differentially expressed proteins are principally involved in antibiotic resistance and linked to oxidative stress response, to DNA protection and to membrane permeability. Moreover, since enrofloxacin is an inhibitor of DNA gyrase, the overexpression of DNA starvation/stationary phase protection protein (Dsp) could be a central point to discover themechanismof this clone to counteract the effects of enrofloxacin. In parallel, the dramatic decrease of the synthesis of the outer membrane proteinW, which represents one of the main gates for enrofloxacin entrance, could explain additional mechanismof E. coli defense against this antibiotic.
Original languageEnglish
Pages (from-to)365-376
Number of pages12
Publication statusPublished - 2015


  • Antibiotic resistance
  • Enrofloxacin
  • Escherichia coli
  • Urinary tract infections


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