Mapping B-cell epitopes of hepatitis C virus E2 glycoprotein using human monoclonal antibodies from phage display libraries

Francesca Bugli, Antonio Gasbarrini, Guido Fadda, Maria Elisabetta Clementi, N Mancini, Cy Kang, Cristiana Di Campli, Antonella Grieco, A Manzin, Armando Gabrielli, Pe Varaldo, R Burioni

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Clinical and experimental evidence indicates that the hepatitis C virus (HCV) E2 glycoprotein (HCV/E2) is the most promising candidate for the development of an effective anti-HCV vaccine. Identification of the human epitopes that are conserved among isolates and are able to elicit protective antibodies would constitute a significant step forward. This work describes the mapping of the B-cell epitopes present on the surface of HCV/E2, as recognized by the immune system during infection, by the analysis of the reciprocal interactions of a panel of human recombinant Fabs derived from an HCV-infected patient. Three unrelated epitopes recognized by antibodies with no neutralization-of-binding (NOB) activity were identified; a fourth, major epitope was defined as a clustering of minor epitopes recognized by Fabs endowed with strong NOB activity.
Original languageEnglish
Pages (from-to)9986-9990
Number of pages5
JournalJournal of Virology
Volume75
DOIs
Publication statusPublished - 2001

Keywords

  • Antibodies, Monoclonal
  • B-Lymphocytes
  • Epitope Mapping
  • Hepacivirus
  • Hepatitis C
  • Hepatitis C Antigens
  • Viral Envelope Proteins

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