Macrophage expression and prognostic significance of the long pentraxin PTX3 in COVID-19

Enrico Brunetta; Marco Folci; Barbara Bottazzi; Maria De Santis; Giuseppe Gritti; Alessandro Protti; Sarah N Mapelli; Stefanos Bonovas; Daniele Piovani; Roberto Leone; Ilaria My; Veronica Zanon; Gianmarco Spata; Monica Bacci; Domenico Supino; Silvia Carnevale; Marina Sironi; Sadaf Davoudian; Clelia Peano; Francesco Landi; Fabiano Di Marco; Federico Raimondi; Andrea Gianatti; Claudio Angelini; Alessandro Rambaldi; Cecilia Garlanda; Michele Ciccarelli; Maurizio Cecconi; Alberto Mantovani

doi:10.1038/s41590-020-00832-x

Macrophage expression and prognostic significance of the long pentraxin PTX3 in COVID-19

Enrico Brunetta
, Marco Folci
, Barbara Bottazzi
, Maria De Santis
, Giuseppe Gritti
, Alessandro Protti
, Sarah N Mapelli
, Stefanos Bonovas
, Daniele Piovani
, Roberto Leone
, Ilaria My
, Veronica Zanon
, Gianmarco Spata
, Monica Bacci
, Domenico Supino
, Silvia Carnevale
, Marina Sironi
, Sadaf Davoudian
, Clelia Peano
, Francesco Landi

Fabiano Di Marco, Federico Raimondi, Andrea Gianatti, Claudio Angelini, Alessandro Rambaldi^*, Cecilia Garlanda^*, Michele Ciccarelli^*, Maurizio Cecconi^*, Alberto Mantovani^*

^*Corresponding author

Research output: Contribution to journal › Article

Abstract

Long pentraxin 3 (PTX3) is an essential component of humoral innate immunity, involved in resistance to selected pathogens and in the regulation of inflammation1–3. The present study was designed to assess the presence and significance of PTX3 in Coronavirus Disease 2019 (COVID-19)4–7. RNA-sequencing analysis of peripheral blood mononuclear cells, single-cell bioinformatics analysis and immunohistochemistry of lung autopsy samples revealed that myelomonocytic cells and endothelial cells express high levels of PTX3 in patients with COVID-19. Increased plasma concentrations of PTX3 were detected in 96 patients with COVID-19. PTX3 emerged as a strong independent predictor of 28-d mortality in multivariable analysis, better than conventional markers of inflammation, in hospitalized patients with COVID-19. The prognostic significance of PTX3 abundance for mortality was confirmed in a second independent cohort (54 patients). Thus, circulating and lung myelomonocytic cells and endothelial cells are a major source of PTX3, and PTX3 plasma concentration can serve as an independent strong prognostic indicator of short-term mortality in COVID-19.

Original language	English
Pages (from-to)	19-24
Number of pages	6
Journal	Nature Immunology
Volume	22
Issue number	1
DOIs	https://doi.org/10.1038/s41590-020-00832-x
Publication status	Published - 2021

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology

Keywords

COVID-19 / complications

Access to Document

10.1038/s41590-020-00832-x

Cite this

Brunetta, E., Folci, M., Bottazzi, B., De Santis, M., Gritti, G., Protti, A., Mapelli, S. N., Bonovas, S., Piovani, D., Leone, R., My, I., Zanon, V., Spata, G., Bacci, M., Supino, D., Carnevale, S., Sironi, M., Davoudian, S., Peano, C., ... Mantovani, A. (2021). Macrophage expression and prognostic significance of the long pentraxin PTX3 in COVID-19. Nature Immunology, 22(1), 19-24. https://doi.org/10.1038/s41590-020-00832-x

@article{242818c7913846e79767353f7efe9ac2,

title = "Macrophage expression and prognostic significance of the long pentraxin PTX3 in COVID-19",

abstract = "Long pentraxin 3 (PTX3) is an essential component of humoral innate immunity, involved in resistance to selected pathogens and in the regulation of inflammation1–3. The present study was designed to assess the presence and significance of PTX3 in Coronavirus Disease 2019 (COVID-19)4–7. RNA-sequencing analysis of peripheral blood mononuclear cells, single-cell bioinformatics analysis and immunohistochemistry of lung autopsy samples revealed that myelomonocytic cells and endothelial cells express high levels of PTX3 in patients with COVID-19. Increased plasma concentrations of PTX3 were detected in 96 patients with COVID-19. PTX3 emerged as a strong independent predictor of 28-d mortality in multivariable analysis, better than conventional markers of inflammation, in hospitalized patients with COVID-19. The prognostic significance of PTX3 abundance for mortality was confirmed in a second independent cohort (54 patients). Thus, circulating and lung myelomonocytic cells and endothelial cells are a major source of PTX3, and PTX3 plasma concentration can serve as an independent strong prognostic indicator of short-term mortality in COVID-19.",

keywords = "COVID-19 / complications, COVID-19 / complications",

author = "Enrico Brunetta and Marco Folci and Barbara Bottazzi and \{De Santis\}, Maria and Giuseppe Gritti and Alessandro Protti and Mapelli, \{Sarah N\} and Stefanos Bonovas and Daniele Piovani and Roberto Leone and Ilaria My and Veronica Zanon and Gianmarco Spata and Monica Bacci and Domenico Supino and Silvia Carnevale and Marina Sironi and Sadaf Davoudian and Clelia Peano and Francesco Landi and \{Di Marco\}, Fabiano and Federico Raimondi and Andrea Gianatti and Claudio Angelini and Alessandro Rambaldi and Cecilia Garlanda and Michele Ciccarelli and Maurizio Cecconi and Alberto Mantovani",

year = "2021",

doi = "10.1038/s41590-020-00832-x",

language = "English",

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Brunetta, E, Folci, M, Bottazzi, B, De Santis, M, Gritti, G, Protti, A, Mapelli, SN, Bonovas, S, Piovani, D, Leone, R, My, I, Zanon, V, Spata, G, Bacci, M, Supino, D, Carnevale, S, Sironi, M, Davoudian, S, Peano, C, Landi, F, Di Marco, F, Raimondi, F, Gianatti, A, Angelini, C, Rambaldi, A, Garlanda, C, Ciccarelli, M, Cecconi, M & Mantovani, A 2021, 'Macrophage expression and prognostic significance of the long pentraxin PTX3 in COVID-19', Nature Immunology, vol. 22, no. 1, pp. 19-24. https://doi.org/10.1038/s41590-020-00832-x

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T1 - Macrophage expression and prognostic significance of the long pentraxin PTX3 in COVID-19

AU - Brunetta, Enrico

AU - Folci, Marco

AU - Bottazzi, Barbara

AU - De Santis, Maria

AU - Gritti, Giuseppe

AU - Protti, Alessandro

AU - Mapelli, Sarah N

AU - Bonovas, Stefanos

AU - Piovani, Daniele

AU - Leone, Roberto

AU - My, Ilaria

AU - Zanon, Veronica

AU - Spata, Gianmarco

AU - Bacci, Monica

AU - Supino, Domenico

AU - Carnevale, Silvia

AU - Sironi, Marina

AU - Davoudian, Sadaf

AU - Peano, Clelia

AU - Landi, Francesco

AU - Di Marco, Fabiano

AU - Raimondi, Federico

AU - Gianatti, Andrea

AU - Angelini, Claudio

AU - Rambaldi, Alessandro

AU - Garlanda, Cecilia

AU - Ciccarelli, Michele

AU - Cecconi, Maurizio

AU - Mantovani, Alberto

PY - 2021

Y1 - 2021

N2 - Long pentraxin 3 (PTX3) is an essential component of humoral innate immunity, involved in resistance to selected pathogens and in the regulation of inflammation1–3. The present study was designed to assess the presence and significance of PTX3 in Coronavirus Disease 2019 (COVID-19)4–7. RNA-sequencing analysis of peripheral blood mononuclear cells, single-cell bioinformatics analysis and immunohistochemistry of lung autopsy samples revealed that myelomonocytic cells and endothelial cells express high levels of PTX3 in patients with COVID-19. Increased plasma concentrations of PTX3 were detected in 96 patients with COVID-19. PTX3 emerged as a strong independent predictor of 28-d mortality in multivariable analysis, better than conventional markers of inflammation, in hospitalized patients with COVID-19. The prognostic significance of PTX3 abundance for mortality was confirmed in a second independent cohort (54 patients). Thus, circulating and lung myelomonocytic cells and endothelial cells are a major source of PTX3, and PTX3 plasma concentration can serve as an independent strong prognostic indicator of short-term mortality in COVID-19.

AB - Long pentraxin 3 (PTX3) is an essential component of humoral innate immunity, involved in resistance to selected pathogens and in the regulation of inflammation1–3. The present study was designed to assess the presence and significance of PTX3 in Coronavirus Disease 2019 (COVID-19)4–7. RNA-sequencing analysis of peripheral blood mononuclear cells, single-cell bioinformatics analysis and immunohistochemistry of lung autopsy samples revealed that myelomonocytic cells and endothelial cells express high levels of PTX3 in patients with COVID-19. Increased plasma concentrations of PTX3 were detected in 96 patients with COVID-19. PTX3 emerged as a strong independent predictor of 28-d mortality in multivariable analysis, better than conventional markers of inflammation, in hospitalized patients with COVID-19. The prognostic significance of PTX3 abundance for mortality was confirmed in a second independent cohort (54 patients). Thus, circulating and lung myelomonocytic cells and endothelial cells are a major source of PTX3, and PTX3 plasma concentration can serve as an independent strong prognostic indicator of short-term mortality in COVID-19.

KW - COVID-19 / complications

UR - https://publicatt.unicatt.it/handle/10807/304078

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U2 - 10.1038/s41590-020-00832-x

DO - 10.1038/s41590-020-00832-x

M3 - Article

SN - 1529-2908

VL - 22

SP - 19

EP - 24

JO - Nature Immunology

JF - Nature Immunology

IS - 1

ER -

Macrophage expression and prognostic significance of the long pentraxin PTX3 in COVID-19

Abstract

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