M tuberculosis in the adjuvant modulates time of appearance of CNS-specific effector T cells in the spleen through a polymorphic site of TLR2

Gabriele Di Sante, Giovanni Delogu, Francesco Ria, Annabella Procoli, Giuseppe Migliara, Alessia Piermattei, Mariagrazia Valentini, Achille Renato Maria Cittadini, Gabriela Constantin

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

DC deliver information regulating trafficking of effector T cells along T-cell priming. However, the role of pathogen-derived motives in the regulation of movement of T cells has not been studied. We hereinafter report that amount of M tuberculosis in the adjuvant modulates relocation of PLP139-151 specific T cells. In the presence of a low dose of M tuberculosis in the adjuvant, T cells (detected by CDR3 BV-BJ spectratyping, the so-called "immunoscope") mostly reach the spleen by day 28 after immunization ("late relocation") in the SJL strain, whereas T cells reach the spleen by d 14 with a high dose of M tuberculosis ("early relocation"). The C57Bl/6 background confers a dominant "early relocation" phenotype to F1 (SJL×C57Bl/6) mice, allowing early relocation of T cells in the presence of low dose M tuberculosis. A single non-synonymous polymorphism of TLR2 is responsible for "early/late" relocation phenotype. Egress of T lymphocytes is regulated by TLR2 expressed on T cells. Thus, pathogens engaging TLR2 on T cells regulate directly T-cell trafficking, and polymorphisms of TLR2 condition T-cell trafficking upon a limiting concentration of ligand.
Original languageEnglish
Pages (from-to)e55819-e55819
JournalPLoS One
Volume8
DOIs
Publication statusPublished - 2013

Keywords

  • CD44
  • T Cells
  • TLR2
  • Trafficking

Fingerprint

Dive into the research topics of 'M tuberculosis in the adjuvant modulates time of appearance of CNS-specific effector T cells in the spleen through a polymorphic site of TLR2'. Together they form a unique fingerprint.

Cite this